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On-line Access: 2021-08-28
Received: 2021-04-21
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Utility ofTPP?manufactured biophysical restrictions toprobe multiscale cellular dynamics
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PanyuFei, HaiboDing, YuDuan, XinyiWang, WeiHu, PengWu, MengxiaoWei, ZhengchunPeng. Utility ofTPP?manufactured biophysical restrictions toprobe multiscale cellular dynamics[J]. Journal of Zhejiang University Science D, 2021, 4(4): 776-789.
@article{title="Utility ofTPP?manufactured biophysical restrictions toprobe multiscale cellular dynamics",
author="PanyuFei, HaiboDing, YuDuan, XinyiWang, WeiHu, PengWu, MengxiaoWei, ZhengchunPeng",
journal="Journal of Zhejiang University Science D",
volume="4",
number="4",
pages="776-789",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-021-00163-2"
}
%0 Journal Article
%T Utility ofTPP?manufactured biophysical restrictions toprobe multiscale cellular dynamics
%A PanyuFei
%A HaiboDing
%A YuDuan
%A XinyiWang
%A WeiHu
%A PengWu
%A MengxiaoWei
%A ZhengchunPeng
%J Journal of Zhejiang University SCIENCE D
%V 4
%N 4
%P 776-789
%@ 1869-1951
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-021-00163-2
TY - JOUR
T1 - Utility ofTPP?manufactured biophysical restrictions toprobe multiscale cellular dynamics
A1 - PanyuFei
A1 - HaiboDing
A1 - YuDuan
A1 - XinyiWang
A1 - WeiHu
A1 - PengWu
A1 - MengxiaoWei
A1 - ZhengchunPeng
J0 - Journal of Zhejiang University Science D
VL - 4
IS - 4
SP - 776
EP - 789
%@ 1869-1951
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-021-00163-2
Abstract: Biophysical restrictions regulate protein difusion, nucleus deformation, and cell migration, which are all universal and
important processes for cells to perform their biological functions. However, current technologies addressing these multiscale questions are extremely limited. Herein, through two-photon polymerization (TPP), we present the precise, low-cost,
and multiscale microstructures (micro-fences) as a versatile investigating platform. With nanometer-scale printing resolution and multiscale scanning capacity, TPP is capable of generating micro-fences with sizes of 0.51000?m. These microfences are utilized as biophysical restrictions to determine the fuidity of supported lipid bilayers (SLB), to investigate the
restricted difusion of Src family kinase protein Lck on SLB, and also to reveal the mechanical bending of cell nucleus and
T cell climbing ability. Taken together, the proposed versatile and low-cost micro-fences have great potential in probing the
restricted dynamics of molecules, organelles, and cells to understand the basics of physical biology.
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