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On-line Access: 2022-09-02
Received: 2022-02-25
Revision Accepted: 2022-07-20
Crosschecked: 2022-09-02
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Rapid and mass manufacturing of soft hydrogel microstructures for cell patterns assisted by 3D printing
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Chaofan He, Xuechun Chen, Yuan Sun, Mingjun Xie, Kang Yu, Jing He, Jinwei Lu, Qing Gao, Jing Nie, Yi Wang & Yong He. Rapid and mass manufacturing of soft hydrogel microstructures for cell patterns assisted by 3D printing[J]. Journal of Zhejiang University Science D, 2022, 5(4): 641-659.
@article{title="Rapid and mass manufacturing of soft hydrogel microstructures for cell patterns assisted by 3D printing",
author="Chaofan He, Xuechun Chen, Yuan Sun, Mingjun Xie, Kang Yu, Jing He, Jinwei Lu, Qing Gao, Jing Nie, Yi Wang & Yong He",
journal="Journal of Zhejiang University Science D",
volume="5",
number="4",
pages="641-659",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-022-00207-1"
}
%0 Journal Article
%T Rapid and mass manufacturing of soft hydrogel microstructures for cell patterns assisted by 3D printing
%A Chaofan He
%A Xuechun Chen
%A Yuan Sun
%A Mingjun Xie
%A Kang Yu
%A Jing He
%A Jinwei Lu
%A Qing Gao
%A Jing Nie
%A Yi Wang & Yong He
%J Journal of Zhejiang University SCIENCE D
%V 5
%N 4
%P 641-659
%@ 1869-1951
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-022-00207-1
TY - JOUR
T1 - Rapid and mass manufacturing of soft hydrogel microstructures for cell patterns assisted by 3D printing
A1 - Chaofan He
A1 - Xuechun Chen
A1 - Yuan Sun
A1 - Mingjun Xie
A1 - Kang Yu
A1 - Jing He
A1 - Jinwei Lu
A1 - Qing Gao
A1 - Jing Nie
A1 - Yi Wang & Yong He
J0 - Journal of Zhejiang University Science D
VL - 5
IS - 4
SP - 641
EP - 659
%@ 1869-1951
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-022-00207-1
Abstract: Micro-/nano-patterns on hydrogels are widely used in cell patterning. However, manufacturing molds with traditional lithography is time-consuming and expensive. In addition, the excessive demolding force can easily damage patterns since biocompatible hydrogels are ultra-soft or brittle. Here, we presented a novel method for rapid and mass fabrication of cell patterns. High-precision three-dimensional (3D) printing was used to manufacture a mold with a resolution of 2 μm, and the gelatin-based hydrogel was cured by thermal–photo-crosslinking so that the low-concentration and low-substitution-rate hydrogel could be demolded successfully. We found that pre-cooling before illumination made gelatin-based hydrogels resilient due to the partial regain of triple-helix structures . With this method, arbitrarily customized hydrogel patterns with a feature size of 6–80 μm can be fabricated stably and at low cost. When cardiomyocytes were seeded on ultra-soft hydrogels with parallel groove structures, a consistent and spontaneous beating with 216 beats per minute (BPM) could be observed, approaching the natural beating rate of rat hearts (300 BPM). Overall, this work provides a general scheme for manufacturing cell patterns which has great potential for cell ethology and tissue repair.
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