CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2024-05-20
Cited: 0
Clicked: 300
I. A. O. Beeren, G. Dos Santos, P. J. Dijkstra, C. Mota, J. Bauer, H. Ferreira, Rui L. Reis, N. Neves, S. Camarero-Espinosa, M. B. Baker & L. Moroni. A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications[J]. Journal of Zhejiang University Science D, 2024, 7(3): 277-291.
@article{title="A facile strategy for tuning the density of surface-grafted biomolecules
for melt extrusion-based additive manufacturing applications",
author="I. A. O. Beeren, G. Dos Santos, P. J. Dijkstra, C. Mota, J. Bauer, H. Ferreira, Rui L. Reis, N. Neves, S. Camarero-Espinosa, M. B. Baker & L. Moroni",
journal="Journal of Zhejiang University Science D",
volume="7",
number="3",
pages="277-291",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-024-00286-2"
}
%0 Journal Article
%T A facile strategy for tuning the density of surface-grafted biomolecules
for melt extrusion-based additive manufacturing applications
%A I. A. O. Beeren
%A G. Dos Santos
%A P. J. Dijkstra
%A C. Mota
%A J. Bauer
%A H. Ferreira
%A Rui L. Reis
%A N. Neves
%A S. Camarero-Espinosa
%A M. B. Baker & L. Moroni
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 3
%P 277-291
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-024-00286-2
TY - JOUR
T1 - A facile strategy for tuning the density of surface-grafted biomolecules
for melt extrusion-based additive manufacturing applications
A1 - I. A. O. Beeren
A1 - G. Dos Santos
A1 - P. J. Dijkstra
A1 - C. Mota
A1 - J. Bauer
A1 - H. Ferreira
A1 - Rui L. Reis
A1 - N. Neves
A1 - S. Camarero-Espinosa
A1 - M. B. Baker & L. Moroni
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 3
SP - 277
EP - 291
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-024-00286-2
Abstract: Melt extrusion-based additive manufacturing (ME-AM) is a promising technique to fabricate porous scaffolds for tissue engineering applications. However, most synthetic semicrystalline polymers do not possess the intrinsic biological activity required
to control cell fate. Grafting of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;
however, there are limited strategies available to control the surface density. Here, we report a strategy to tune the surface density
of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k (PCL5k) containing orthogonally reactive azide
groups with an unfunctionalized high molecular weight PCL75k at different ratios. Stable porous three-dimensional (3D) scaffolds were then fabricated using a high weight percentage (75 wt.%) of the low molecular weight PCL5k. As a proof-of-concept
test, we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted
with an alkynated fluorescent model compound on the surface, yielding a density of 201–561 pmol/cm2. Subsequently, a bone
morphogenetic protein 2 (BMP-2)-derived peptide was grafted onto the films comprising different blend compositions, and the
effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells (hMSCs) was assessed.
After two weeks of culturing in a basic medium, cells expressed higher levels of BMP receptor II (BMPRII) on films with the
conjugated peptide. In addition, we found that alkaline phosphatase activity was only significantly enhanced on films containing the highest peptide density (i.e., 561 pmol/cm2), indicating the importance of the surface density. Taken together, these
results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material interface.
Moreover, we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality
via blending of (modified) polymers. Furthermore, the use of alkyne–azide “click” chemistry enables spatial control over
bioconjugation of many tissue-specific moieties, making this approach a versatile strategy for tissue engineering applications.
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