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Received: 2007-12-11

Revision Accepted: 2008-02-04

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.4 P.279-285

http://doi.org/10.1631/jzus.B0730029


A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen


Author(s):  Cheng ZHOU, Xiao-dong KANG, Zhi CHEN

Affiliation(s):  Institute of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   zch8@hotmail.com, Chenzhi@zju.edu.cn

Key Words:  Toll-like receptor 2 (TLR2), Ligand, Allergic immune response, Mite allergen


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Cheng ZHOU, Xiao-dong KANG, Zhi CHEN. A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen[J]. Journal of Zhejiang University Science B, 2008, 9(4): 279-285.

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author="Cheng ZHOU, Xiao-dong KANG, Zhi CHEN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="4",
pages="279-285",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0730029"
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%T A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen
%A Cheng ZHOU
%A Xiao-dong KANG
%A Zhi CHEN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 4
%P 279-285
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0730029

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T1 - A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen
A1 - Cheng ZHOU
A1 - Xiao-dong KANG
A1 - Zhi CHEN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 4
SP - 279
EP - 285
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0730029


Abstract: 
It has been proposed that activation of Toll-like receptors (TLRs) plays crucial roles in the polarization of adaptive immune responses. A synthetic toll-like receptor 2 (TLR2) ligand, Pam3CSK4, has been reported to modulate the balance of Th1/Th2 responses. We evaluated the modulation effect of Pam3CSK4 on allergic immune response in a mouse rhinitis model sensitized to house dust mite allergen (HDM). Mice were sensitized and challenged with Dermatophagoides farinae allergen (Der f), and then the allergic mice were treated by Pam3CSK4. Nasal allergic symptoms and eosinophils were scored. Der f-specific cytokine responses were examined in the splenocytes and bronchoalveolar lavage fluid (BALF). Serum level of total IgE was also detected. After establishing a mouse allergic rhinitis model with HDM, we have showed that Pam3CSK4 treatment not only ameliorated the nasal allergic symptoms remarkably but also decreased the eosinophils and total inflammation cells in BALF significantly. Analysis of cytokine profile found that IFN-γ released from either BALF or stimulated splenocytes increased markedly in Pam3CSK4-treated mice, while IL-13 decreased significantly. Moreover, serum level of total IgE was significantly lower in Pam3CSK4-treated mice than in the untreated. Thus, in an allergic rhinitis mouse model developed with HDM, Pam3CSK4 was shown to exhibit an antiallergic effect, indicating its potential application in allergic diseases.

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