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CLC number: TB131; Q274

On-line Access: 2011-03-09

Received: 2010-04-23

Revision Accepted: 2010-07-13

Crosschecked: 2011-02-17

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.3 P.210-218


Moisture sorption characteristics of freeze-dried human platelets

Author(s):  Meng-jie Xu, Guang-ming Chen, Ju-li Fan, Jin-hui Liu, Xian-guo Xu, Shao-zhi Zhang

Affiliation(s):  Institute of Refrigeration and Cryogenics, Zhejiang University, Hangzhou 310027, China, College of Aerospace Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China, Key Laboratory of Blood Safety Research, Ministry of Health, Blood Center of Zhejiang Province, Hangzhou 310006, China

Corresponding email(s):   enezsz@zju.edu.cn

Key Words:  Human platelets, Freeze-drying, Sorption, Moisture

Meng-jie Xu, Guang-ming Chen, Ju-li Fan, Jin-hui Liu, Xian-guo Xu, Shao-zhi Zhang. Moisture sorption characteristics of freeze-dried human platelets[J]. Journal of Zhejiang University Science B, 2011, 12(3): 210-218.

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author="Meng-jie Xu, Guang-ming Chen, Ju-li Fan, Jin-hui Liu, Xian-guo Xu, Shao-zhi Zhang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Moisture sorption characteristics of freeze-dried human platelets
%A Meng-jie Xu
%A Guang-ming Chen
%A Ju-li Fan
%A Jin-hui Liu
%A Xian-guo Xu
%A Shao-zhi Zhang
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 3
%P 210-218
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1010199

T1 - Moisture sorption characteristics of freeze-dried human platelets
A1 - Meng-jie Xu
A1 - Guang-ming Chen
A1 - Ju-li Fan
A1 - Jin-hui Liu
A1 - Xian-guo Xu
A1 - Shao-zhi Zhang
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 3
SP - 210
EP - 218
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1010199

freeze-drying is a promising method for a long-term storage of human platelets. The moisture sorption characteristics of freeze-dried human platelets (FDHPs) were studied in this paper. The moisture sorption isotherms of FDHPs and freeze-dried lyophilization buffer (FDLB) were measured at 4, 25, and 37 °C. The experimental data were fitted to Brunauer-Emmett-Teller (BET) and Guggenheim-Anderson-de Boer (GAB) equations. There were no significant statistical differences (P>0.05) between the sorption characteristics of FDHPs and FDLB at 4 and 25 °C, while FDHPs absorbed more water at 37 °C. The net isosteric heat of sorption was derived. The heat for FDHPs showed an abnormal negative value at low moisture contents when 25 and 37 °C data were used. Dynamic sorption experiments were carried out at 25 °C with environmental water activity controlled at 0.75, 0.85, and 0.90. The moisture diffusion coefficient was fitted to be 8.24×10−12 m2/s when experimental data at initial time were used. These results would be helpful in choosing prehydration and storage condition for FDHPs.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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