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CLC number: S811

On-line Access: 2015-06-08

Received: 2014-11-30

Revision Accepted: 2015-05-16

Crosschecked: 2015-05-25

Cited: 9

Clicked: 5444

Citations:  Bibtex RefMan EndNote GB/T7714


Xiu-qi Wang


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.549-559


Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells

Author(s):  Chun-qi Gao, Yin-ling Zhao, Hai-chang Li, Wei-guo Sui, Hui-chao Yan, Xiu-qi Wang

Affiliation(s):  College of Animal Science, South China Agricultural University / National Engineering Research Center for Breeding Swine Industry / Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, China; more

Corresponding email(s):   yanhc@scau.edu.cn, xqwang@scau.edu.cn

Key Words:  Heat stress, Swine, Cell proliferation, Cell growth, Apoptosis, Akt/mTOR/S6K pathway

Chun-qi Gao, Yin-ling Zhao, Hai-chang Li, Wei-guo Sui, Hui-chao Yan, Xiu-qi Wang. Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells[J]. Journal of Zhejiang University Science B, 2015, 16(6): 549-559.

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author="Chun-qi Gao, Yin-ling Zhao, Hai-chang Li, Wei-guo Sui, Hui-chao Yan, Xiu-qi Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells
%A Chun-qi Gao
%A Yin-ling Zhao
%A Hai-chang Li
%A Wei-guo Sui
%A Hui-chao Yan
%A Xiu-qi Wang
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 6
%P 549-559
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400339

T1 - Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells
A1 - Chun-qi Gao
A1 - Yin-ling Zhao
A1 - Hai-chang Li
A1 - Wei-guo Sui
A1 - Hui-chao Yan
A1 - Xiu-qi Wang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 549
EP - 559
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400339

Proliferation suppression and apoptosis are the prominent characteristics induced by heat stress (HS) in cells, whereas the effects of HS on cell growth (mass accumulation) are unknown. In this study, Lantang swine (an indigenous breed of China) skeletal muscle satellite cells (SCs) were pre-cultured at 37 °C for 24 h. The HS group was subjected to HS at 41 °C, while the control group was maintained at 37 °C. Heat shock protein 70 (HSP70) expression and SC size are significantly increased (P<0.05) by HS, but cell proliferation is suppressed (P<0.05) and apoptosis is induced (P<0.05). HS led to a lower percentage of SCs in the G0/G1 phase (P<0.05) together with a higher percentage of SCs in the S phase (P<0.05). However, the percentage of SCs in the G2/M phase was decreased (P<0.05) at 48 h but then increased (P<0.05) at 72 h with HS. In addition, the phosphorylation ratios of protein kinase b (Akt), ribosomal protein S6 kinase (S6K), and ribosomal protein S6 were increased (P<0.05) by HS. Nevertheless, the phosphorylation ratios of the 4E binding protein 1 and the eukaryotic initiation factor-4E were indistinguishable (P>0.05) from those of the control group. The phosphorylation ratio of the mammalian target of rapamycin (mTOR) (Ser2448) increased (P<0.05) within 48 h, and apparent differences were abrogated at 72 h (P>0.05). Moreover, cleaved caspase-3 expression was increased at 72 h (P<0.05). These findings indicate that HS induces apoptosis and disrupts cell cycle distribution to decrease the number of cells. Additionally, HS can promote SC growth via an activated Akt/mTOR/S6K signaling pathway.




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