CLC number: R73-37
On-line Access: 2016-07-06
Received: 2015-11-04
Revision Accepted: 2016-01-22
Crosschecked: 2016-06-18
Cited: 2
Clicked: 4099
Citations: Bibtex RefMan EndNote GB/T7714
Jiong-huang Chen, Jian-yang Xiang, Guo-ping Ding, Li-ping Cao. Cholangiocarcinoma-derived exosomes inhibit the antitumor activity of cytokine-induced killer cells by down-regulating the secretion of tumor necrosis factor-α and perforin[J]. Journal of Zhejiang University Science B, 2016, 17(7): 537-544.
@article{title="Cholangiocarcinoma-derived exosomes inhibit the antitumor activity of cytokine-induced killer cells by down-regulating the secretion of tumor necrosis factor-α and perforin",
author="Jiong-huang Chen, Jian-yang Xiang, Guo-ping Ding, Li-ping Cao",
journal="Journal of Zhejiang University Science B",
volume="17",
number="7",
pages="537-544",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500266"
}
%0 Journal Article
%T Cholangiocarcinoma-derived exosomes inhibit the antitumor activity of cytokine-induced killer cells by down-regulating the secretion of tumor necrosis factor-α and perforin
%A Jiong-huang Chen
%A Jian-yang Xiang
%A Guo-ping Ding
%A Li-ping Cao
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 7
%P 537-544
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500266
TY - JOUR
T1 - Cholangiocarcinoma-derived exosomes inhibit the antitumor activity of cytokine-induced killer cells by down-regulating the secretion of tumor necrosis factor-α and perforin
A1 - Jiong-huang Chen
A1 - Jian-yang Xiang
A1 - Guo-ping Ding
A1 - Li-ping Cao
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 7
SP - 537
EP - 544
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500266
Abstract: Objective: The aim of our study is to observe the impact of cholangiocarcinoma-derived exosomes on the antitumor activities of cytokine-induced killer (CIK) cells and then demonstrate the appropriate mechanism. Methods: tumor-derived exosomes (TEXs), which are derived from RBE cells (human cholangiocarcinoma line), were collected by ultracentrifugation. CIK cells induced from peripheral blood were stimulated by TEXs. Fluorescence-activated cell sorting (FACS) was performed to determine the phenotypes of TEX-CIK and N-CIK (normal CIK) cells. The concentrations of tumor necrosis factor-α (TNF-α) and perforin in the culture medium supernatant were examined by using an enzyme-linked immunosorbent assay (ELISA) kit. A CCK-8 kit was used to evaluate the cytotoxic activity of the CIK cells to the RBE cell line. Results: The concentrations of TNF-α and perforin of the group TEX-CIK were 138.61 pg/ml and 2.41 ng/ml, respectively, lower than those of the group N-CIK 194.08 pg/ml (P<0.01) and 3.39 ng/ml (P<0.05). The killing rate of the group TEX-CIK was 33.35%, lower than that of the group N-CIK (47.35% (P<0.01)). The population of CD3+, CD8+, NK (CD56+), and CD3+CD56+ cells decreased in the TEX-CIK group ((63.2±6.8)%, (2.5±1.0)%, (0.53±0.49)%, (0.45±0.42)%) compared with the N-CIK group ((90.3±7.3)%, (65.7±3.3)%, (4.2±1.2)%, (15.2±2.7)%), P<0.01. Conclusions: Our results suggest that RBE cells-derived exosomes inhibit the antitumor activity of CIK cells by down-regulating the population of CD3+, CD8+, NK (CD56+), and CD3+CD56+ cells and the secretion of TNF-α and perforin. TEX may play an important role in cholangiocarcinoma immune escape.
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