|
Journal of Zhejiang University SCIENCE A
ISSN 1673-565X(Print), 1862-1775(Online), Monthly
2002 Vol.3 No.2 P.216-222
cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus
Abstract: Genome segments S8 of two Chinese isolates of rice black-streaked dwarf virus (RBSDV), one from Zhejiang Province and another from Hebei Province, were amplified by RT-PCR and sequenced. Both segments consisted of 1936 nts in full length (EMBL accession numbers were AJ297431 and AJ297432, respectively) and contained only one big open reading frame which encoded a polypeptide with molecular weight of 68kD. The two Chinese isolates shared 94.0% and 96.5% identity at nucleotide and amino acid level, respectively. They shared 94.5-94.9% and 92.5-92.9% homology with S8 of RBSDV Japanese isolate at nucleotide and amino acid level, respectively; shared 85.1-87.6% and 91.7-91.9% homology with S7 of Italian MRDV (maize rough dwarf virus).
Key words: Rice black-streaked dwarf virus(RBSDV); Genome segment S8, Sequence analysis, Biotechnology
References:
Open peer comments: Debate/Discuss/Question/Opinion
<1>
DOI:
10.1631/jzus.2002.0216
CLC number:
S432.41
Download Full Text:
Downloaded:
2922
Clicked:
5770
Cited:
27
On-line Access:
Received:
2001-07-18
Revision Accepted:
2001-11-29
Crosschecked: