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Journal of Zhejiang University SCIENCE A
ISSN 1673-565X(Print), 1862-1775(Online), Monthly
2003 Vol.4 No.3 P.331-335
Cloning and characterization of a glucose 6-phosphate/phosphate translocator from Oryza sativa
Abstract: Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator (GPT) was isolated from a cDNA library of immature seeds of rice and named as OsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of 70 amino acid residues. The OsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression of OsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported via GPT can be used for starch biosynthesis in rice nongreen plastids.
Key words: Glucose 6-phosphate/phosphate translocator, Starch synthesis, Rice (Oryza sativa L.)
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DOI:
10.1631/jzus.2003.0331
CLC number:
Q943.2
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Received:
2002-06-26
Revision Accepted:
2002-09-06
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