|
Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2005 Vol.6 No.2 P.132-136
Representative appressorium stage cDNA library of Magnaporthe grisea
Abstract: A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a λTriplEx2 vector by SMARTTM cDNA library containing 2.37×106 independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.
Key words: Magnaporthe grisea, Appressorium, cDNA library
References:
Open peer comments: Debate/Discuss/Question/Opinion
<1>
DOI:
10.1631/jzus.2005.B0132
CLC number:
Q949.32
Download Full Text:
Downloaded:
3056
Clicked:
6679
Cited:
14
On-line Access:
Received:
2004-04-29
Revision Accepted:
2004-06-15
Crosschecked: