Abstract: RNA methyltransferase is responsible for transferring methyl and resulting in methylation on the bases or ribose ring of RNA, which existed widely but mostly remains an open question. A recombinant protein PH1948 predicting RNA methyltransferase from Pyrococcus horikoshii OT3 has been crystallized. The crystals of selenomethionyl PH1948 belong to space group C2, with unit-cell parameters a=207.0 Å, b=43.1 Å, c=118.2 Å, β=92.1°, and diffract X-rays to 2.2 Å resolution. The V_M value was determined to be 2.8 ų/Da, indicating the presence of four protein molecules in the asymmetric unit.

Key words: Pyrococcus horikoshii, Methyltransferase, X-ray diffraction, Protein crystallography

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