Abstract: Waste hops are good sources of flavonoids. Extraction of flavonoids from waste hops (SC-CO₂ extracted hops) using supercritical fluids technology was investigated. Various temperatures, pressures and concentrations of ethanol (modifier) and the ratio (w/w) of solvent to material were tested in this study. The results of single factor and orthogonal experiments showed that at 50 °C, 25 MPa, the ratio of solvent to material (50%), ethanol concentration (80%) resulted in maximum extraction yield flavonoids (7.8 mg/g). HPLC-MS analysis of the extracts indicated that flavonoids obtained were xanthohumol, the principal prenylflavonoid in hops.

Key words: Optimization, Supercritical fluid extraction, Flavonoids, Xanthohumol, Hops (Humulus lupulus L.)

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