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Journal of Zhejiang University SCIENCE B

ISSN 1673-1581(Print), 1862-1783(Online), Monthly

2007 Vol.8 No.9 P.661-665

Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea

LU Jian-ping, DUAN Zhi-bing, LIU Tong-bao, LIN Fu-cheng

Department of Biology, School of Life Sciences, Zhejiang University, Hangzhou 310058, China; Biotechnology Institute, Zhejiang University, Hangzhou 310029, China

jplu@zju.edu.cn; fuchenglin@zju.edu.cn

Abstract: The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the “TATA” box, the “CAAT” box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences indicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.

Key words: Magnaporthe grisea, Promoter, NAR, Clone, Hypha


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DOI:

10.1631/jzus.2007.B0661

CLC number:

S432; Q933

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Received:

2007-05-08

Revision Accepted:

2007-06-22

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