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Journal of Zhejiang University SCIENCE B

ISSN 1673-1581(Print), 1862-1783(Online), Monthly

Differential expression of salt tolerance related genes in Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee

Abstract: We examined salt tolerance responsive genes in Pak-choi under salt stress and analyze their potential function. The mRNA differential display was used to screen the transcript derived fragments (TDFs) related to salinity tolerance in tolerant and moderately tolerant Pak-choi germplasm. Seventy-eight primer combinations generated 101 differential cDNA fragments, which were divided into 10 expression types. Seven cDNA sequences (GenBank accession Nos. DQ006915~DQ006921) obtained and sequenced were highly homologous to some known expression genes or the genes related to the signaling pathways in plants under different abiotic stress.

Key words: Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee, Salinity tolerance, Gene differential expression, cDNA fragments, Basic local alignment search tool (BLAST) analysis


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DOI:

10.1631/jzus.B0920098

CLC number:

S63; Q37

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Received:

2009-04-09

Revision Accepted:

2009-08-23

Crosschecked:

2009-10-13

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