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Journal of Zhejiang University SCIENCE B

ISSN 1673-1581(Print), 1862-1783(Online), Monthly

Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: applications of phytoremediation

Abstract: A hydroponics experiment was aimed at identifying the lead (Pb) tolerance and phytoremediation potential of Moso bamboo (Phyllostachys pubescens) seedlings grown under different Pb treatments. Experimental results indicated that at the highest Pb concentration (400 μmol/L), the growth of bamboo seedlings was inhibited and Pb concentrations in leaves, stems, and roots reached the maximum of 148.8, 482.2, and 4282.8 mg/kg, respectively. Scanning electron microscopy revealed that the excessive Pb caused decreased stomatal opening, formation of abundant inclusions in roots, and just a few inclusions in stems. The ultrastructural analysis using transmission electron microscopy revealed that the addition of excessive Pb caused abnormally shaped chloroplasts, disappearance of endoplasmic reticulum, shrinkage of nucleus and nucleolus, and loss of thylakoid membranes. Although ultrastructural analysis revealed some internal damage, even the plants exposed to 400 µmol/L Pb survived and no visual Pb toxicity symptoms such as necrosis and chlorosis were observed in these plants. Even at the highest Pb treatment, no significant difference was observed for the dry weight of stem compared with controls. It is suggested that use of Moso bamboo as an experimental material provides a new perspective for remediation of heavy metal contaminated soil owing to its high metal tolerance and greater biomass.

Key words: Moso bamboo, Pb, Phytoremediation, Scanning electron microscopy, Transmission electron microscopy

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目的:探索毛竹在修å¤é“…污染土壤的æ¤ç‰©ä¿®å¤æ½œåŠ›ã€‚
创新点:使用了毛竹作为一ç§å…¨æ–°çš„æ¤ç‰©ä¿®å¤ææ–™è¿›è¡Œç ”ç©¶ã€‚å¾—åˆ°äº†æ¯›ç«¹å¹¼è‹—åœ¨é“…èƒè¿«ä¸‹çš„生长与生ç†å应情况,ä¸åŒæ¤ç‰©ç»„ç»‡å¯¹é“…çš„å¸æ”¶ä¸Žç§¯ç´¯æƒ…况,铅èƒè¿«ä¸‹æ¯›ç«¹å¹¼è‹—表层与细胞超微结构的特å¾ã€‚
方法:根系形æ€åˆ†æžï¼ˆå›¾3ï¼‰ã€æ¤ç‰©é‡é‡‘属å«é‡åˆ†æž (表1ï¼‰ã€æ‰«æç”µé•œåˆ†æžï¼ˆå›¾4)和é€å°„电镜分æžï¼ˆå›¾5)。
结论:å„组织部ä½é“…浓度的值æ­ç¤ºäº†ï¼Œæ¯›ç«¹æ˜¯ä¸€ä¸ªå¾ˆå¥½çš„固定铅污染土壤的æ¤ç‰©ææ–™ï¼›ä½†æ˜¯ç”±äºŽæ¯›ç«¹èŒŽçš„高生物é‡ï¼Œä¹Ÿå¯ä»¥ä½œä¸ºé“…污染土壤的æ¤ç‰©ä¿®å¤ææ–™ã€‚在0~200μmol/L铅处ç†ä¸‹ï¼Œæ¯›ç«¹çš„生物é‡ä¸Žæ ¹ç³»å½¢æ€æ²¡æœ‰æ˜¾è‘—性差异,生长正常未出现中毒症状。通过扫æç”µé•œè§‚察铅èƒè¿«å¯¼è‡´å¶ç‰‡çš„æ°”å­”å‡å°‘,根与茎内å«ç‰©å¢žå¤šã€‚通过é€å°„电镜观察高浓度的铅èƒè¿«å¯¼è‡´éƒ¨åˆ†ç»†èƒžå†…部å¶ç»¿ä½“å˜å½¢ã€å†…质网消失ã€ç»†èƒžæ ¸ä¸Žæ ¸ä»ç¼©å°å’Œç±»å›Šä½“膜消失。尽管超微结构观察到细胞内部的æŸå®³ï¼Œä½†æ˜¯æœªå‡ºçŽ°è§†è§‰çš„æ¯’æ€§ç—‡çŠ¶ã€‚æ¯›ç«¹é“…çš„é«˜è€æ€§ä¸ŽèŒŽçš„高生物é‡ï¼Œæä¾›äº†æ¯›ç«¹ä½œä¸ºææ–™ä¿®å¤åœŸå£¤é‡é‡‘属铅更大的å¯èƒ½æ€§ã€‚

关键è¯ç»„:毛竹;æ¤ç‰©ä¿®å¤ï¼›é“…;扫æç”µé•œï¼›é€å°„电镜


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DOI:

10.1631/jzus.B1400107

CLC number:

X173

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On-line Access:

2024-08-27

Received:

2023-10-17

Revision Accepted:

2024-05-08

Crosschecked:

2015-01-07

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