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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2015 Vol.16 No.7 P.632-639
Correlation analysis of gene polymorphisms and β-lactam allergy
Abstract: A total of 64 patients with β-lactam allergy and 30 control subjects were enrolled in a case-control study. This study is aimed to analyze the relationship between β-lactam allergy and 10 single nucleotide polymorphisms (SNPs) in interleukin-10 (IL-10), IL-13, IL-4Rα, high-affinity immunoglobulin E-receptor β chain (FcεRIβ), interferon γ receptor 2 (IFNGR2), and CYP3A4, and within the Han Chinese population of Northwest China. Genotyping for the SNPs was conducted using the Sequenom MassARRAY® platform. SPSS 17.0 was employed to analyze the statistical data and SHEsis was used to perform the haplotype reconstruction and analyze linkage disequilibrium of SNPs of IL-10 and IL-13. The results showed that the genotype distribution of CYP3A4 rs2242480/CT differed significantly between case and control groups of males (P=0.022; odds ratio (OR)=0.167, 95% confidence interval (CI): 0.032–0.867). Further analysis showed that CCA, CCG, and TAA haplotypes of IL-10 had no significant correlation in patients with β-lactam allergy. The correlation between CCT and CAC haplotypes of IL-13 and β-lactam allergy needs to be further studied. The analysis did not reveal any differences in the distribution of others gene polymorphisms between cases and controls.
Key words: Allergy, β-Lactam, Interleukin (IL), Pharmacogenomics, Single nucleotide polymorphism (SNP)
Chinese Summary <45> 基因多态性与β-内酰胺类抗生素过敏的关联性分析
创新点：首次在甘肃地区汉族人群中探讨10个单核苷酸多态性位点与β-内酰胺类抗生素过敏易感性的关联性，为该地区人群β-内酰胺类抗生素过敏患者提供了基因组学水平的诊断数据。
方法：以β-内酰胺类抗生素过敏者为研究对象进行病例对照研究，采用Sequenom MassARRAY®分子量阵列技术平台定制单核苷酸多态性（SNP）芯片检测10个单核苷酸多态性与甘肃地区汉族人群β-内酰胺类抗生素过敏易感性的关联性。应用SHEsis软件完成IL-10及IL-13的连锁不平衡分析及单倍型构建。
结论：CYP3A4 rs2242480基因多态性与男性患者β-内酰胺类抗生素过敏有显著的相关性（P=0.022；OR=0.167，95% CI：0.032-0.867）。IL-13的CCT及CAC单倍型与中国西北地区汉族β-内酰胺类抗生素过敏的相关性有待进一步研究。
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DOI:
10.1631/jzus.B1400309
CLC number:
R394.6
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On-line Access:
2024-08-27
Received:
2023-10-17
Revision Accepted:
2024-05-08
Crosschecked:
2015-06-16
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