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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2017 Vol.18 No.7 P.615-625
Effects on cytotoxicity and antibacterial properties of the incorporations of silver nanoparticles into the surface coating of dental alloys
Abstract: The aim of this study was to research the changes in cytotoxicity and antibacterial properties after silver nanoparticles (AgNPs) were incorporated into the surface coating of dental alloys. AgNPs were attached to cobalt chromium alloys and pure titanium using a hydrothermal method, according to the reaction: AgNO3+NaBH4→ Ag+1/2H2+1/2B2H6+NaNO3. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of the alloys when in contact with osteogenic precursor cells (MC3T3-E1) from mice and mesenchymal stem cells (BMSC) from rats. The antibacterial properties of dental alloys incorporating three different concentrations (10, 4, and 2 μmol/L) of AgNPs were tested on Staphylococcus aureus (SA) and Streptococcus mutans (MS). High cytotoxicity values were observed for all dental alloys that contained 0% of AgNPs (the control groups). The incorporation of AgNPs reduced cytotoxicity values. No significant difference was observed for antibacterial performance when comparing dental alloys containing AgNPs to the respective control groups. The results demonstrated that the cobalt chromium alloys and pure titanium all had cytotoxicity to MC3T3-E1 and BMSC and that the incorporation of AgNPs could reduce this cytotoxicity. The concentrations of AgNPs adopted in this study were found to have no antibacterial action against SA or MS.
Key words: Silver nanoparticles (AgNPs); Dental casting; Cytotoxicity; Antibacterial; MC3T3-E1; BMSC
创新点:è¿ç”¨MTT法è¯å®žé’´é“¬åˆé‡‘和纯钛对å°é¼ æˆéª¨å‰ä½“细胞(MC3T3-E1)åŠå¤§é¼ 骨髓间充质干细胞(BMSC)产生细胞毒性,粘附纳米银颗粒åŽç»†èƒžæ¯’性有所é™ä½Žã€‚
方法:将化å¦æ³•åˆ¶å¾—çš„3ç§æµ“度的纳米银颗粒分别粘附于6ç§ç‰™ç§‘åˆé‡‘表é¢ï¼Œæ‰«æ电镜观察并确认纳米银的粘附情况。采用MTT法检测ä¸åŒæµ“度纳米银颗粒的牙科åˆé‡‘对MC3T3-E1åŠBMSC的细胞毒性。评价3ç§æµ“度纳米银颗粒的钴铬åˆé‡‘和纯钛试件浸æ液对金黄色葡è„çƒèŒå’Œå˜å½¢é“¾çƒèŒçš„抗èŒæ€§ã€‚
结论:牙科åˆé‡‘对MC3T3-E1å’ŒBMSC细胞具有较强的毒性,粘附纳米银颗粒åŽç»†èƒžæ¯’性有所é™ä½Žã€‚3ç§æµ“åº¦çš„çº³ç±³é“¶é¢—ç²’ç»†èƒžæ¯’æ€§ä¹‹é—´æ— æ˜¾è‘—æ€§å·®å¼‚ï¼Œä¸”è¿™3ç§æµ“度纳米银颗粒粘附åŽå¯¹ç‰™ç§‘åˆé‡‘的抗èŒæ€§æ— 明显影å“。
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DOI:
10.1631/jzus.B1600555
CLC number:
R783.1
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On-line Access:
2017-07-05
Received:
2016-12-08
Revision Accepted:
2017-03-22
Crosschecked:
2017-06-16