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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2018 Vol.19 No.6 P.425-435
NF-κB in mitochondria regulates PC12 cell apoptosis following lipopolysaccharide-induced injury
Abstract: Objective: To determine the relationship between nuclear transcription factor κB (NF-κB) expression in mitochondria and neuronal cell apoptosis after lipopolysaccharide (LPS)-induced injury. Methods: The effect of drug administration on PC12 cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and Hoechst 33342 staining. The morphology and function of mitochondria were investigated with electron microscopy and rhodamine 123, respectively. The activity of adenine nucleotide translocase 1 (ANT1), lipid peroxide, and anti-peroxidase enzymes was measured by enzyme-linked immunosorbent assay (ELISA). Relative expression levels of NF-κB were measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Results: Pyrrolidine dithiocarbamate (PDTC, an NF-κB inhibitor) and nerve growth factor (NGF) not only reduced apoptosis but also had a protective effect on mitochondrial structure and function in a PC12 cell LPS damage model. The subcellular distribution of NF-κB demonstrated that NGF, diterpene acid atractyloside (ATR, an ANT1 antagonist), and PDTC alleviated increases in mitochondrial NF-κB after LPS-induced injury. Conclusions: (1) NF-κB is activated in mitochondria via uptake of ANT1 during apoptosis following LPS-induced injury in neuronal cells; (2) NGF not only decreases the activity of NF-κB but also reduces ANT1 activity, which in turn decreases NF-κB levels in mitochondria and suppresses mitochondria-mediated apoptosis.
Key words: Nuclear transcription factor κB (NF-κB); Mitochondria; Apoptosis; Adenine nucleotide translocase 1 (ANT1); Lipopolysaccharide (LPS)
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References:
[1]Amigoni L, Martegani E, Colombo S, 2013. Lack of HXK2 induces localization of active Ras in mitochondria and triggers apoptosis in the yeast Saccharomyces cerevisiae. Oxid Med Cell Longev, 2013:678473.
[2]Bayunova LV, Parnova GG, Avrova NF, 2015. Antiapoptotic effect of gangliosides on PC12 cells exposed to bacterial lipopolysaccharide. Zh Evol Biokhim Fiziol, 51(2):88-94 (in Russian).
[3]Cardoso S, Santos RX, Correia SC, et al., 2013. Insulin-induced recurrent hypoglycemia exacerbates diabetic brain mitochondrial dysfunction and oxidative imbalance. Neurobiol Dis, 49:1-12.
[4]Carrillo-de Sauvage MA, Maatouk L, Arnoux I, et al., 2013. Potent and multiple regulatory actions of microglial glucocorticoid receptors during CNS inflammation. Cell Death Differ, 20:1546-1572.
[5]Cheng Q, Yang G, Ma J, et al., 2014. Effects of different types of fluid resuscitation on hepatic mitochondria and apoptosis. Exp Ther Med, 7(2):335-342.
[6]Christensen ME, Jansen ES, Sanchez W, et al., 2013. Flow cytometry based assays for the measurement of apoptosis-associated mitochondrial membrane depolarisation and cytochrome c release. Methods, 61(2):138-145.
[7]Gavaldà-Navarro A, Villena JA, Planavila A, et al., 2014. Expression of adenine nucleotide translocase (ANT) isoform genes is controlled by PGC-1α through different transcription factors. J Cell Physiol, 229(12):2126-2136.
[8]Ghavami S, Shojaei S, Yeganeh B, et al., 2014. Autophagy and apoptosis dysfunction in neurodegenerative disorders. Prog Neurobiol, 112:24-49.
[9]Hernandez-Esquivel L, Pavón N, Buelna-Chontal M, et al., 2014. Citicoline (CDP-choline) protects myocardium from ischemia/reperfusion injury via inhibiting mitochondrial permeability transition. Life Sci, 96(1-2):53-58.
[10]Hirata I, Yasumoto S, Toshina K, et al., 2007. Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis. World J Gastroenterol, 13(11):1666-1671.
[11]Kerr JF, Wyllie AH, Currie AR, 1972. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer, 26(4):239-257.
[12]Long TY, Jing R, Kuang F, et al., 2017. CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway. Braz J Med Biol Res, 50(4):5861-5868.
[13]Lu TH, Tseng TJ, Su CC, et al., 2014. Arsenic induces reactive oxygen species-caused neuronal cell apoptosis through JNK/ERK-mediated mitochondria-dependent and GRP 78/CHOP-regulated pathways. Toxicol Lett, 224(1):130-140.
[14]Lyu A, Chen JJ, Wang HC, et al., 2017. Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 18(6):481-491.
[15]Mao DS, Song BW, Zhu XH, et al., 2011. The protective effect of nerve growth factor on PC12 cells to lipopolysaccharide injury. Chin J Appl Physiol, 27(1):93-97 (in Chinese).
[16]Mariappan N, Elks CM, Sriramula S, et al., 2010. NF-κB-induced oxidative stress contributes to mitochondrial and cardiac dysfunction in type II diabetes. Cardiovasc Res, 85(3):473-483.
[17]Que RS, Lin C, Ding GP, et al., 2016. Increasing the immune activity of exosomes: the effect of miRNA-depleted exosome proteins on activating dendritic cell/cytokine-induced killer cells against pancreatic cancer. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 17(5):352-360.
[18]Sapsrithong T, Kaewprem W, Tongumpai S, et al., 2012. Cissus quadrangularis ethanol extract upregulates superoxide dismutase, glutathione peroxidase and endothelial nitric oxide synthase expression in hydrogen peroxide-injured human ECV304 cells. J Ethnopharmacol, 143(2):664-672.
[19]Song Y, Xiao Y, Wang JM, et al., 2014. The different molecular mechanisms of mitophagy between yeast and mammals. Crit Rev Eukaryot Gene Expr, 24(1):29-38.
[20]Song Y, Ding W, Xiao Y, et al., 2015. The progress of mitophagy and related pathogenic mechanisms of the neurodegenerative diseases and tumor. Neurosci J, 2015: 543758.
[21]Sugawara T, Chan PH, 2003. Reactive oxygen radicals and pathogenesis of neuronal death after cerebral ischemia. Antioxid Redox Signal, 5(5):597-607.
[22]Wani WY, Sunkaria A, Sharma DR, et al., 2014. Caspase inhibition augments Dichlorvos-induced dopaminergic neuronal cell death by increasing ROS production and PARP1 activation. Neuroscience, 258:1-15.
[23]Xu W, Zhou Q, Yao Y, et al., 2016. Inhibitory effect of Gardenblue blueberry (Vaccinium ashei Reade) anthocyanin extracts on lipopolysaccharide-stimulated inflammatory response in RAW 264.7 cells. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 17(6):425-436.
[24]Xu Y, Xue Y, Wang Y, et al., 2009. Multiple-modulation effects of Oridonin on the production of proinflammatory cytokines and neurotrophic factors in LPS-activated microglia. Int Immunopharmacol, 9(3):360-365.
[25]Ye J, Liu Z, Wei J, 2013. Protective effect of SIRT1 on toxicity of microglial-derived factors induced by LPS to PC12 cells via the p53-caspase-3-dependent apoptotic pathway. Neurosci Lett, 553:72-77.
[26]Zamora M, Meroño C, Viñas O, et al., 2004. Recruitment of NF-κB into mitochondria is involved in adenine nucleotide translocase 1 (ANT1)-induced apoptosis. J Biol Chem, 279(37):38415-38423.
[27]Zhivotovsky B, Galluzzi L, Kepp O, et al., 2009. Adenine nucleotide translocase: a component of the phylogenetically conserved cell death machinery. Cell Death Differ, 16(11):1419-1425.
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DOI:
10.1631/jzus.B1700488
CLC number:
R34
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On-line Access:
2018-06-04
Received:
2017-09-26
Revision Accepted:
2018-03-29
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2018-05-29