Publishing Service

Polishing & Checking

Journal of Zhejiang University SCIENCE B

ISSN 1673-1581(Print), 1862-1783(Online), Monthly

Drying temperature affects rice seed vigor via gibberellin, abscisic acid, and antioxidant enzyme metabolism

Abstract: Seed vigor is a key factor affecting seed quality. The mechanical drying process exerts a significant influence on rice seed vigor. The initial moisture content (IMC) and drying temperature are considered the main factors affecting rice seed vigor through mechanical drying. This study aimed to determine the optimum drying temperature for rice seeds according to the IMC, and elucidate the mechanisms mediating the effects of drying temperature and IMC on seed vigor. Rice seeds with three different IMCs (20%, 25%, and 30%) were dried to the target moisture content (14%) at four different drying temperatures. The results showed that the drying temperature and IMC had significant effects on the drying performance and vigor of the rice seeds. The upper limits of drying temperature for rice seeds with 20%, 25%, and 30% IMCs were 45, 42, and 38 °C, respectively. The drying rate and seed temperature increased significantly with increasing drying temperature. The drying temperature, drying rate, and seed temperature showed extremely significant negative correlations with germination energy (GE), germination rate, germination index (GI), and vigor index (VI). A high IMC and drying temperature probably induced a massive accumulation of hydrogen peroxide (H2O2) and superoxide anions in the seeds, enhanced superoxide dismutase (SOD) and catalase (CAT) activity, and increased the abscisic acid (ABA) content. In the early stage of seed germination, the IMC and drying temperature regulated seed germination through the metabolism of H2O2, gibberellin acid (GA), ABA, and α-amylase. These results indicate that the metabolism of reactive oxygen species (ROS), antioxidant enzymes, GA, ABA, and α-amylase might be involved in the mediation of the effects of drying temperature on seed vigor. The results of this study provide a theoretical basis and technical guidance for the mechanical drying of rice seeds.

Key words: Drying temperature; Rice; Seed vigor; Gibberellin acid (GA); Abscisic acid (ABA); Antioxidant enzyme

Chinese Summary  <24> 干燥温度通过赤霉素、脱落酸和抗氧化酶代谢影响水稻种子活力

关键词组:干燥温度;水稻;种子活力;赤霉素;脱落酸;抗氧化酶


Share this article to: More

Go to Contents

References:

<HIDE>

[1]Aquerreta J, Iguaz A, Arroqui C, et al., 2007. Effect of high temperature intermittent drying and tempering on rough rice quality. J Food Eng, 80(2):611-618.

[2]Barba-Espín G, Diaz-Vivancos P, Job D, et al., 2011. Understanding the role of H2O2 during pea seed germination: a combined proteomic and hormone profiling approach. Plant Cell Environ, 34(11):1907-1919.

[3]Bie WB, Srzednick G, Driscoll RH, 2007. Study of temperature and moisture distribution in paddy in a triangular spouted bed dryer. Dry Technol, 25(1):177-183.

[4]Boccaccini A, Lorrai R, Ruta V, et al., 2016. The DAG1 transcription factor negatively regulates the seed-to-seedling transition in Arabidopsis acting on ABA and GA levels. BMC Plant Biol, 16:198.

[5]Cao DD, Wu W, Chen SY, et al., 2019. Seed vigor testing by low temperature germination in early rice. J Zhejiang Univ (Agric Life Sci), 45(6):657-666 (in Chinese).

[6]Dang XJ, Thi TGT, Dong GS, et al., 2014. Genetic diversity and association mapping of seed vigor in rice (Oryza sativa L.). Planta, 239(6):1309-1319.

[7]Doymaz I, 2007. Air-drying characteristics of tomatoes. J Food Eng, 78(4):1291-1297.

[8]Doymaz I, 2008. Convective drying kinetics of strawberry. Chem Eng Process Process Intensificat, 47(5):914-919.

[9]Du H, Wu N, Chang Y, et al., 2013. Carotenoid deficiency impairs ABA and IAA biosynthesis and differentially affects drought and cold tolerance in rice. Plant Mol Biol, 83(4-5):475-488.

[10]Endoh K, Matsushita M, Kimura MK, et al., 2018. Cryopreservation of Fagus crenata seeds: estimation of optimum moisture content for maintenance of seed viability by Bayesian modeling. Can J For Res, 48(2):192-196.

[11]Gao CH, Hu J, Zhang S, et al., 2009. Association of polyamines in governing the chilling sensitivity of maize genotypes. Plant Growth Regul, 57(1):31-38.

[12]Gawrysiak-Witulska M, Siger A, Rudzińska M, et al., 2019. The effect of drying on the native tocopherol and phytosterol content of Sinapis alba L. seeds. J Sci Food Agric, 100(1):354-361.

[13]Hasan AAM, Bala BK, Rowshon MK, 2014. Thin layer drying of hybrid rice seed. Eng Agric Environ Food, 7(4):169-175.

[14]He YQ, Cheng JP, Liu LF, et al., 2015. Effects of pre-harvest chemical application on rice desiccation and seed quality. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 16(10):813-823.

[15]Holdsworth MJ, Finch-Savage WE, Grappin P, et al., 2008. Post-genomics dissection of seed dormancy and germination. Trends Plant Sci, 13(1):7-13.

[16]Hu QJ, Fu YY, Guan YJ, et al., 2016. Inhibitory effect of chemical combinations on seed germination and pre-harvest sprouting in hybrid rice. Plant Growth Regul, 80(3):281-289.

[17]Huang AX, She XP, Cao BH, et al., 2011. Distribution of hydrogen peroxide during adventitious roots initiation and development in mung bean hypocotyls cuttings. Plant Growth Regul, 64(2):109-118.

[18]Huang L, Hong YB, Zhang HJ, et al., 2016. Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance. BMC Plant Biol, 16:203.

[19]Huang YT, Lin C, He F, et al., 2017. Exogenous spermidine improves seed germination of sweet corn via involvement in phytohormone interactions, H2O2 and relevant gene expression. BMC Plant Biol, 17:1.

[20]Huang YT, Cao DD, Zou WX, et al., 2020. Study on issue and solution of rice seed mechanical drying in Zhejiang Province. China Rice, 26(3):91-95 (in Chinese).

[21]Hussain S, Zheng MM, Khan F, et al., 2015. Benefits of rice seed priming are offset permanently by prolonged storage and the storage conditions. Sci Rep, 5:8101.

[22]Igathinathane C, Chattopadhyay PK, Pordesimo LO, 2008. Moisture diffusion modeling of parboiled paddy accelerated tempering process with extended application to multi-pass drying simulation. J Food Eng, 88(2):239-253.

[23]Ji XM, Dong BD, Shiran B, et al., 2011. Control of abscisic acid catabolism and abscisic acid homeostasis is important for reproductive stage stress tolerance in cereals. Plant Physiol, 156(2):647-662.

[24]Jiang MY, Zhang JH, 2001. Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol, 42(11):1265-1273.

[25]Jittanit W, Srzednicki G, Driscoll R, 2010a. Corn, rice, and wheat seed drying by two-stage concept. Dry Technol, 28(6):807-815.

[26]Jittanit W, Srzednicki G, Driscoll R, 2010b. Seed drying in fluidized and spouted bed dryers. Dry Technol, 28(10):1213-1219.

[27]Kalemba EM, Pukacka S, 2014. Carbonylated proteins accumulated as vitality decreases during long-term storage of beech (Fagus sylvatica L.) seeds. Trees, 28(2):503-515.

[28]Kim SK, Son TK, Park SY, et al., 2006. Influences of gibberellin and auxin on endogenous plant hormone and starch mobilization during rice seed germination under salt stress. J Environ Biol, 27(2):181-186.

[29]Li Z, Peng Y, Zhang XQ, et al., 2014. Exogenous spermidine improves seed germination of white clover under water stress via involvement in starch metabolism, antioxidant defenses and relevant gene expression. Molecules, 19(11):18003-18024.

[30]Liu JK, Xie M, Li XZ, et al., 2018. Main allelochemicals from the rhizosphere soil of Saussurea lappa (Decne.) Sch. Bip. and their effects on plants’ antioxidase systems. Molecules, 23(10):2506.

[31]Madamba PS, Yabes RP, 2005. Determination of the optimum intermittent drying conditions for rough rice (Oryza sativa, L.). Food Sci Technol, 38(2):157-165.

[32]Musielak G, 2000. Influence of the drying medium parameters on drying induced stresses. Dry Technol, 18(3):561-581.

[33]Nguyen DN, Lee KJ, Kim DL, et al., 2014. Modeling and validation of high-temperature induced spikelet sterility in rice. Field Crop Res, 156:293-302.

[34]Pieruzzi FP, Dias LLC, Balbuena TS, et al., 2011. Polyamines, IAA and ABA during germination in two recalcitrant seeds: Araucaria angustifolia (Gymnosperm) and Ocotea odorifera (Angiosperm). Ann Bot, 108(2):337-345.

[35]Qiu J, Wang RM, Yan JZ, et al., 2005. Seed film coating with uniconazole improves rape seedling growth in relation to physiological changes under waterlogging stress. Plant Growth Regul, 47(1):75-81.

[36]Rajjou L, Duval M, Gallardo K, et al., 2012. Seed germination and vigor. Ann Rev Plant Biol, 63:507-533.

[37]Ratajczak E, Małecka A, Bagniewska-Zadworna A, et al., 2015. The production, localization and spreading of reactive oxygen species contributes to the low vitality of long-term stored common beech (Fagus sylvatica L.) seeds. J Plant Physiol, 174:147-156.

[38]Soliman WS, Fujimori M, Tase K, et al., 2011. Oxidative stress and physiological damage under prolonged heat stress in C3 grass Lolium perenne. Grassl Sci, 57(2):101-106.

[39]Souza GFMV, Miranda RF, Barrozo MAS, 2015. Soybean (Glycine max L. Merrill) seed drying in fixed bed: process heterogeneity and seed quality. Dry Technol, 33(14):1779-1787.

[40]Su LQ, Li JG, Xue H, et al., 2017. Super absorbent polymer seed coatings promote seed germination and seedling growth of Caragana korshinskii in drought. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 18(8):696-706.

[41]Tangney R, Merritt DJ, Fontaine JB, et al., 2019. Seed moisture content as a primary trait regulating the lethal temperature thresholds of seeds. J Ecol, 107(3):1093-1105.

[42]Thakur AK, Gupta AK, 2006. Two stage drying of high moisture paddy with intervening rest period. Energ Convers Manage, 47(18-19):3069-3083.

[43]Uddin Z, Suppakul P, Boonsupthip W, 2016. Effect of air temperature and velocity on moisture diffusivity in relation to physical and sensory quality of dried pumpkin seeds. Dry Technol, 34(12):1423-1433.

[44]Ueno K, 2003. Effects of temperature during drying of immature wheat seed on germination. Seed Sci Technol, 31(3):587-595.

[45]https://doi.org/10.15258/sst.2003.31.3.08

[46]Vishwakarma K, Upadhyay N, Kumar N, et al., 2017. Abscisic acid signaling and abiotic stress tolerance in plants: a review on current knowledge and future prospects. Front Plant Sci, 20(8):161.

[47]Wang F, Cheng FM, Liu Y, et al., 2006. Dynamic changes of plant hormones in developing grains at rice filling stage under different temperatures. Acta Agron Sin, 32(1):25-29 (in Chinese).

[48]Wang F, Qi JZ, Zheng XG, et al., 2017. Effects of artificial aging on seed vigor, starch decomposition and endogenous hormones of barley in the early germination. Xinjiang Agric Sci, 54(1):33-42 (in Chinese).

[49]Yamauchi Y, Ogawa M, Kuwahara A, et al., 2004. Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds. Plant Cell, 16(2):367-378.

[50]Ye YY, Liu YM, Ji FL, et al., 2003. The present situation and development of crop seed dryers. J Chin Agric Mech, 41(5):22-24 (in Chinese).

[51]Zhang JP, Wang HY, Liao SX, et al., 2019. Appropriate ultra-low seed moisture content stabilizes the seed longevity of Calocedrus macrolepis, associated with changes in endogenous hormones, antioxidant enzymes, soluble sugars and unsaturated fatty acids. New For, 50(3):455-468.

[52]Zhao YJ, Wang T, 2001. Analysis of the relationship between α-amylase and germinating rate of rice seeds during the process of seed germination. Chin Bull Bot, 18(2):226-230 (in Chinese).

[53]Zhou XX, Liu L, Fu PC, et al., 2018. Effects of infrared radiation drying and heat pump drying combined with tempering on the quality of long-grain paddy rice. Int J Food Sci Tech, 53(11):2448-2456.

[54]List of electronic supplementary materials

[55]Table S1 Effects of drying temperature on seed germination energy (GE), germination percentage (GP), germination index (GI) and vigor index (VI) of rice stored for 10 months

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





DOI:

10.1631/jzus.B2000297

CLC number:

S351.1

Download Full Text:

Click Here

Downloaded:

2017

Download summary:

<Click Here> 

Downloaded:

1618

Clicked:

3710

Cited:

0

On-line Access:

2020-10-12

Received:

2020-06-03

Revision Accepted:

2020-07-12

Crosschecked:

2020-09-09

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952276; Fax: +86-571-87952331; E-mail: jzus@zju.edu.cn
Copyright © 2000~ Journal of Zhejiang University-SCIENCE