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Journal of Zhejiang University SCIENCE B

ISSN 1673-1581(Print), 1862-1783(Online), Monthly

Arthrospira maxima (Spirulina) prevents endoplasmic reticulum stress in the kidney through its C-phycocyanin

Abstract: Arthrospira maxima (Spirulina) is a cyanobacterium which is considered a nutraceutical because it has antioxidant, anti-inflammatory, and cytoprotective properties in different renal disease models (Rodriguez-Sánchez et al., 2012; Aziz et al., 2018; Memije-Lazaro et al., 2018). The therapeutic effects are due to the presence of metabolites with biological effects similar to those of essential fatty acids ω-3 and ω-6, vitamins A, C and E, and accessory pigments such as phycobiliproteins. One of the most abundant phycobiliproteins in A. maxima is C-phycocyanin (Mysliwa-Kurdziel and Solymosi, 2017). This molecule is responsible for nephroprotective action in a model of acute kidney injury (AKI) because it reduces oxidative stress and caspase activation (Rodriguez-Sánchez et al., 2012; Rojas-Franco et al., 2018). However, both A. maxima and its C-phycocyanin are related to the reduction of the redox environment. Thus, they probably help to maintain the adequate function of the intracellular organelles like the endoplasmic reticulum. However, this therapeutic action has not been evaluated previously.

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Chinese Summary  <31> 极大节螺藻(螺旋藻)C-藻蓝蛋白阻碍肾脏内质网胁迫

概要:极大节螺藻(Arthrospira maxima)细胞内含有C-藻蓝蛋白,其具有抗氧化和细胞保护作用,因此该藻全细胞和C-藻蓝蛋白均被确认为保健品。然而,它们的保健效应尚未被充分认识。由于它们可以降低未折叠蛋白反应,我们推测,极大节螺藻细胞及其C-藻蓝蛋白可以防止肾脏内质网胁迫(ERS)。本文旨在研究极大节螺藻细胞及其C-藻蓝蛋白通过ERS反应对氯化汞(HgCl2)引起的肾损伤的影响。实验公鼠先用极大节螺藻细胞或C-藻蓝蛋白处理30 min,再用5 mgHgCl2/kg体重处理,之后三天均饲喂藻细胞或C-藻蓝蛋白。实验鼠安乐死后,取其肾脏用于分析蛋白激酶RNA样内质网激酶(PERK)、转录激活因子6(ATF-6)和需肌醇酶1α(IRE-1α)信号途径。结果表明,汞中毒会提高PERK/eIF2α/ATF-4/GADD153和IRE-1α/XBP1途径,而极大节螺藻细胞处理防止了PERK、ATF-6α和半胱天冬酶的提高;同时,C-藻蓝蛋白处理使得所有这些蛋白得到正常表达。本研究首次证实了极大节螺藻处理通过其C-藻蓝蛋白的活性防止了HgCl2引发的肾脏ERS。

关键词组:C-藻蓝蛋白;极大节螺藻;螺旋藻;肾脏内质网胁迫;肾保护


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DOI:

10.1631/jzus.B2000725

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On-line Access:

2024-08-27

Received:

2023-10-17

Revision Accepted:

2024-05-08

Crosschecked:

2022-04-22

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