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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2022 Vol.23 No.4 P.345-351
Comparative transcriptome analysis of candidate genes involved in chlorogenic acid biosynthesis during fruit development in three pear varieties of Xinjiang Uygur Autonomous Region
Abstract: Pear is one of the main fruits with thousands of years of cultivation history in China. There are more than 2000 varieties of pear cultivars around the world, including more than 1200 varieties or cultivars in China (Legrand et al., 2016). Xinjiang Uygur Autonomous Region is an important pear production region in China with 30 of varieties or cultivars. Pyrus sinkiangensis is the most popular variety, which is mainly distributed in Xinjiang (Zhou et al., 2018). Chlorogenic acid (CGA), p-coumaric acid, and arbutin are the main polyphenols in pear fruit, and their levels show great differences among different varieties (Li et al., 2014). CGA is a potential chemo-preventive agent, which possesses many important bioactivities including antioxidant, diabetes attenuating, and anti-obesity (Wang et al., 2021). Therefore, the specific CGA content of a variety is considered the embodiment of the functional nutritional value of pears.
Key words: Pear varieties; Chlorogenic acid; Transcriptome analysis; DEGs; WGCNA
机构：1浙江大学，热工与动力系统研究所，中国杭州，310027；2浙江大学，能源清洁利用国家重点实验室，中国杭州，310027；3中国科学院，南京土壤研究所，中国南京，210008
目的：本文旨在探究多种物性参数（含水率、干容积密度、粒径分布和化学组成）对充分压实（相对压实度接近100%，绝对压实度大于85%）的回填土热导率的影响规律，并提出便于工程上使用的压实回填土热导率的预测公式。
创新点：通过普氏击实试验（落锤法）使各土壤试样的相对压实度均接近100%，以模拟压实回填土的实际工况，从而使测得的热导率数据和预测模型能够为工程提供可靠的参考。
方法：1.通过实验测试，探究含水率、干容积密度、粒径分布和化学组成于充分压实回填土热导率的影响规律。2.通过回归分析，提出基于质地分类的压实回填土热导率的预测公式。3.预测首先通过相关性分析确定的对土壤热导率影响程度最大的两个参数。
结论：1.压实回填土热导率的最大值比其他文献所报道的自然状态或非压实土壤热导率的最大值大20%~50%。2.与容积干密度类似，压实回填土的热导率随含水率的升高并非一直单调增大；当含水率超过某临界值时，热导率会基本保持不变或缓慢减小，且热导率的峰值点对应的临界含水率一般略大于容积干密度的峰值点对应的临界含水率。3.总体而言，随含水率的升高、干容积密度的增大、粒径的增大、石英含量的升高和有机质含量的降低，压实回填土的热导率均增大，且含水率和干容积密度对热导率的影响较其他因素更显著。4.基于含水率和干容积密度的1/2次方的二元线性回归得到的压实回填土热导率的预测公式具有较高的置信度（各项回归系数的P值均小于0.05）和拟合精度（复相关系数R2大于0.9）。
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DOI:
10.1631/jzus.B2100673
CLC number:
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On-line Access:
2022-04-11
Received:
2021-08-01
Revision Accepted:
2021-12-19
Crosschecked:
2022-04-19