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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2022 Vol.23 No.11 P.943-956
Combination of Se-methylselenocysteine, D-α-tocopheryl succinate, β-carotene, and L-lysine can prevent cancer metastases using as an adjuvant therapy
Abstract: ObjectivePrimary tumor treatment through surgical resection and adjuvant therapy has been extensively studied, but there is a lack of effective strategies and drugs for the treatment of tumor metastases. Here, we describe a functional product based on a combination of compounds, which can be used as an adjuvant therapy and has well-known mechanisms for inhibiting cancer metastases, improving anti-cancer treatment, and enhancing immunity and antioxidant capacity. Our designed combination, named MVBL, consists of four inexpensive compounds: L-selenium-methylselenocysteine (MSC), D-α-tocopheryl succinic acid (VES),β-carotene (β-Ca), and L-lysine (Lys).
MethodsThe effects of MVBL on cell viability, cell cycle, cell apoptosis, cell migration, cell invasion, reactive oxygen species (ROS), and paclitaxel (PTX)-combined treatment were studied in vitro. The inhibition of tumor metastasis, antioxidation, and immune enhancement capacity of MVBL were determined in vivo.
ResultsMVBL exhibited higher toxicity to tumor cells than to normal cells. It did not significantly affect the cell cycle of cancer cells, but increased their apoptosis. Wound healing, adhesion, and transwell assays showed that MVBL significantly inhibited tumor cell migration, adhesion, and invasion. MVBL sensitized MDA-MB-231 breast cancer cells to PTX, indicating that it can be used as an adjuvant to enhance the therapeutic effect of chemotherapy drugs. In mice, experimental data showed that MVBL inhibited tumor metastasis, prolonged their survival time, and enhanced their antioxidant capacity and immune function.
ConclusionsThis study revealed the roles of MVBL in improving immunity and antioxidation, preventing tumor growth, and inhibiting metastasis in vitro and in vivo. MVBL may be used as an adjuvant drug in cancer therapy for improving the survival and quality of life of cancer patients.
Key words: Cancer prevention; Cancer metastases; Selenium (Se); Combination of drugs; Nutrition
1闽江学院材料与化学工程学院智能药物闽台港澳科技合作基地，福建福州，350108
2福州大学化学学院，肿瘤转移预警与预防研究中心，福建省肿瘤转移化学预防重点实验室，福建福州，350002
3闽江学院福州海洋研究院，福建福州，350108
目的：通过手术切除和辅助疗法治疗原发性肿瘤已被广泛研究，但对于肿瘤转移的有效治疗策略和药物仍然缺乏。本研究中，我们提出了一种基于组合化合物的功能性产品用作肿瘤辅助治疗，各化合物机制明确，可抑制癌症转移、改善抗癌治疗、增强免疫力和抗氧化。该组合由四种廉价化合物组成：
方法：通过体外实验研究MVBL对细胞活力、细胞周期、细胞凋亡、细胞迁移、细胞侵袭、活性氧（ROS）和紫杉醇（PTX）联合治疗的影响。在动物水平测定了MVBL在体内的抗氧化和免疫增强能力。通过小鼠肿瘤模型测定MVBL在体内对肿瘤转移的抑制。
结果：MVBL对肿瘤细胞的毒性高于对正常细胞的毒性。它没有显著性地影响癌细胞的细胞周期，但增加了细胞凋亡。划痕、粘附和侵袭实验结果表明，MVBL显著抑制肿瘤细胞迁移、粘附和侵袭。MVBL使乳腺癌MDA-MB-231细胞对PTX更敏感，表明它可以作为佐剂来增强化疗药物的治疗效果。小鼠实验数据表明，MVBL可以抑制肿瘤转移，延长其生存时间，增强其抗氧化能力和免疫功能。
结论：本研究揭示了MVBL在提高免疫、抗氧化、防止肿瘤生长和抑制体内外转移方面的作用。MVBL可用作癌症治疗的辅助药物，提高癌症患者的生存率和生活质量。
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DOI:
10.1631/jzus.B2200232
CLC number:
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On-line Access:
2024-08-27
Received:
2023-10-17
Revision Accepted:
2024-05-08
Crosschecked:
2022-11-16