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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2023 Vol.24 No.5 P.418-429
Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines
Abstract: Efforts have been made to establish various human pluripotent stem cell lines. However, such methods have not yet been duplicated in non-human primate cells. Here, we introduce a multiplexed single-cell sequencing technique to profile the molecular features of monkey pluripotent stem cells in published culture conditions. The results demonstrate suboptimized maintenance of pluripotency and show that the selected signaling pathways for resetting human stem cells can also be interpreted for establishing monkey cell lines. Overall, this work legitimates the translation of novel human cell line culture conditions to monkey cells and provides guidance for exploring chemical cocktails for monkey stem cell line derivation.
Key words: Monkey pluripotent stem cell; Multiplexed single-cell sequencing; Naive pluripotency; Extended pluripotent stem cells
1昆明理工大学， 省部共建非人灵长类生物医学国家重点实验室， 中国昆明市， 650500
2昆明理工大学生命科学与技术学院， 中国昆明市， 650500
3云南省灵长类生物医学重点实验室， 中国昆明市， 650500
4生物岛实验室， 中国广州市， 510005
5广州国家实验室， 中国广州市， 510005
摘要：尽管人们已经开发了多种构建人类多能干细胞的方法，但这些方法仍未应用在非人灵长类细胞中。在本文中，我们利用多样品标记单细胞测序技术对不同培养条件下的猴多能干细胞进行了分子特征的表征。结果表明，虽然用于重置人类干细胞的信号通路可以用来构建猴干细胞系，但其配方有待优化才能更好地维持猴干细胞多能性。总而言之，本研究证实了新型的人类干细胞培养体系可以转化到猴干细胞体系中，这对于未来猴干细胞系培养配方的探索具有指导意义。
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DOI:
10.1631/jzus.B2200440
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On-line Access:
2023-05-15
Received:
2022-09-04
Revision Accepted:
2023-01-15
Crosschecked:
2023-05-16