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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2023 Vol.24 No.10 P.922-934
Cellulose nanofibril matrix drives the dynamic formation of spheroids
Abstract: Multicellular spheroids, which mimic the natural organ counterparts, allow the prospect of drug screening and regenerative medicine. However, their application is hampered by low processing efficiency or limited scale. This study introduces an efficient method to drive rapid multicellular spheroid formation by a cellulose nanofibril matrix. This matrix enables the facilitated growth of spheroids (within 48 h) through multiple cell assembly into size-controllable aggregates with well-organized physiological microstructure. The efficiency, dimension, and conformation of the as-formed spheroids depend on the concentration of extracellular nanofibrils, the number of assembled cells, and the heterogeneity of cell types. The above strategy allows the robust formation mechanism of compacted tumoroids and hepatocyte spheroids.
Key words: Cellulose; Nanofibril; Matrix; Self-assembly; Spheroid
1浙江大学化学工程与生物工程学院， 中国杭州市， 310027
2浙江大学杭州国际科创中心， 中国杭州市， 311215
3上海科技大学物质科学与技术学院， 中国上海市， 201210
摘要：模仿天然组织器官的类器官多细胞微球在药物筛选和再生医学等领域具有广泛前景。然而，多细胞微球技术面临一些挑战，例如低加工效率或规模限制等。本研究介绍了一种通过纳米纤维素基质快速驱动形成多细胞球体的方法。该方法能够快速促进多个细胞组装形成尺寸可控的多细胞微球（48小时），形成具有类似组织的生理微观结构和特征。所形成的微球的效率、尺寸和构象取决于纳米纤维素的浓度、组装细胞的数量和细胞类型的异质性。该方法可以稳定促进肿瘤类器官和肝细胞球状体的高效形成。
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DOI:
10.1631/jzus.B23d0003
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On-line Access:
2023-06-26
Received:
2023-03-16
Revision Accepted:
2023-05-08
Crosschecked:
2023-09-26