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Frontiers of Information Technology & Electronic Engineering

ISSN 2095-9184 (print), ISSN 2095-9230 (online)

Computational methods in super-resolution microscopy

Abstract: The broad applicability of super-resolution microscopy has been widely demonstrated in various areas and disciplines. The optimization and improvement of algorithms used in super-resolution microscopy are of great importance for achieving optimal quality of super-resolution imaging. In this review, we comprehensively discuss the computational methods in different types of super-resolution microscopy, including deconvolution microscopy, polarization-based super-resolution microscopy, structured illumination microscopy, image scanning microscopy, super-resolution optical fluctuation imaging microscopy, single-molecule localization microscopy, Bayesian super-resolution microscopy, stimulated emission depletion microscopy, and translation microscopy. The development of novel computational methods would greatly benefit super-resolution microscopy and lead to better resolution, improved accuracy, and faster image processing.

Key words: Super-resolution microscopy; Deconvolution; Computational methods

Chinese Summary  <22> 超分辨显微成像技术中的计算方法

概要:超分辨显微成像技术已广泛应用于多个领域。其中,计算方法的优化和提升,对超分辨显微系统成像质量优化至关重要。本文综述了目前主流超分辨显微技术采用的计算方法,包括反卷积显微技术、基于偏振的超分辨显微技术、结构光照明显微技术、图像扫描显微技术、超分辨光学涨落显微成像技术、单分子定位显微技术、贝叶斯分析超分辨显微技术、受激辐射损耗显微技术和移位显微技术。新计算方法的发展将有助于提升超分辨显微的成像效果,使其具备更好的分辨率、更高的精度和更快的图像处理速度。

关键词组:超分辨显微;反卷积;计算方法


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DOI:

10.1631/FITEE.1601628

CLC number:

O436

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On-line Access:

2017-10-25

Received:

2016-10-14

Revision Accepted:

2016-12-13

Crosschecked:

2017-09-25

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