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Frontiers of Information Technology & Electronic Engineering

ISSN 2095-9184 (print), ISSN 2095-9230 (online)

XL-RIS empowered near-field physical layer security against jamming and eavesdropping attacks

Abstract: Wireless communication is vulnerable to malicious jamming and eavesdropping attacks due to the broadcast nature of wireless channels. An extremely-large-scale reconfigurable intelligent surface (XL-RIS) demonstrates its abilities to enhance the physical layer security (PLS) and compensate for the severe path loss. We investigate an XL-RIS empowered near-field PLS communication system against jamming and eavesdropping attacks with the help of artificial noise (AN). To maximize the secrecy capacity, we propose an alternating optimization (AO) based algorithm to jointly optimize the beamformers at the base station (BS) and the reflection coefficient matrix at the XL-RIS, subject to the BS’s maximum transmit power and the XL-RIS’s unit-modulus constraints. For the beamforming and AN design at the BS, auxiliary variables are introduced to reformulate the subproblem into a more tractable problem, which is solved by the proposed successive convex approximation (SCA) based algorithm. For the reflection coefficient matrix design at the XL-RIS, a manifold optimization (MO) based algorithm is proposed to address the challenge of large-scale variables and unit-modulus constraints. Numerical results show that XL-RIS can ensure secure communication even if the eavesdropper is located at the same direction as the legitimate user and closer to the XL-RIS.

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Chinese Summary  <19> 联合核糖体工程与培养基优化提高解淀粉芽孢杆菌LX-6中性蛋白酶活性及其在豆粕发酵中的应用

朱怡凡1, 黄心怡1, 韩涛2, 王骥腾2, 俞晓平1, 马正1
1中国计量大学生命科学学院, 国家市场监督管理总局微生物计量检测与生物制品质量安全重点实验室, 中国杭州市, 310018
2浙江海洋大学水产养殖学院, 中国杭州市, 316022
摘要:解淀粉芽孢杆菌(Bacillus amyloliquefaciens LX-6)因具有中性蛋白酶活性,在豆粕(soybean meal, SBM)发酵中具有较大应用潜力。本研究通过核糖体工程及培养基优化技术,提高解淀粉芽孢杆菌LX-6的中性蛋白酶活性。首先,利用核糖体工程技术从306个自发利福平抗性(Rif r)突变株中筛选出突变株5r-10。与野生型(WT)菌株LX-6相比,突变株的中性蛋白酶活性提高了38.45%。研究发现,突变株编码RNA聚合酶β亚基的rpoB基因在第469个氨基酸位点发生谷氨酸→精氨酸(glutamic acid→arginine,Glu→Arg)的突变;在形态上与WT菌株LX-6相比,突变株5r-10的细胞表面更光滑且鞭毛更少。随后,在摇瓶实验中,我们采用基于响应面方法的单因素实验和Box-Behnken设计,对菌株产中性蛋白酶培养基成分进行了优化。在最佳浓度的乳糖、酪蛋白和氯化钙(CaCl2)培养条件下,突变株5r-10的中性蛋白酶活性最高可以达到(3470.67±23.17) U/mL,是WT菌株LX-6在原始培养基中产生的中性蛋白酶活性的1.49倍。此外,与WT菌株LX-6相比,突变株5r-10在固体发酵过程中对SBM中大分子抗原蛋白的降解更快,即与LX-6发酵相比,经突变株5r-10发酵后,SBM中大豆球蛋白和β-伴大豆球蛋白的降解率显著增加。

关键词组:解淀粉芽孢杆菌LX-6;核糖体工程;培养基优化;中性蛋白酶活性;豆粕


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DOI:

10.1631/FITEE.2400477

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On-line Access:

2025-01-24

Received:

2024-06-04

Revision Accepted:

2025-01-24

Crosschecked:

2024-10-10

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