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Journal of Zhejiang University SCIENCE A

ISSN 1673-565X(Print), 1862-1775(Online), Monthly

cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus

Abstract: Genome segments S8 of two Chinese isolates of rice black-streaked dwarf virus (RBSDV), one from Zhejiang Province and another from Hebei Province, were amplified by RT-PCR and sequenced. Both segments consisted of 1936 nts in full length (EMBL accession numbers were AJ297431 and AJ297432, respectively) and contained only one big open reading frame which encoded a polypeptide with molecular weight of 68kD. The two Chinese isolates shared 94.0% and 96.5% identity at nucleotide and amino acid level, respectively. They shared 94.5-94.9% and 92.5-92.9% homology with S8 of RBSDV Japanese isolate at nucleotide and amino acid level, respectively; shared 85.1-87.6% and 91.7-91.9% homology with S7 of Italian MRDV (maize rough dwarf virus).

Key words: Rice black-streaked dwarf virus(RBSDV); Genome segment S8, Sequence analysis, Biotechnology


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DOI:

10.1631/jzus.2002.0216

CLC number:

S432.41

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Received:

2001-07-18

Revision Accepted:

2001-11-29

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