Abstract: Lipophilic tea polyphenols (LTP) were prepared by catalytic esterifica tion of green tea polyphenols (GTP) with hexadecanoyl chloride. A novel long-ch a in acyl-derivative of epigallocatechin-3-o-gallate (EGCG) was first isol ated fro m purification of LTP by high-speed countercurrent chromatography (HSCCC) using a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:1:1:1, v/v). The molecular structure of the acyl-derivative, Epigallocatechin-3-O- gall ate-4'-O-hexadecanate, was elucidated by means of elemental analysis, I R, ¹H-NMR and MS spectra.

Key words: Green tea polyphenols, Lipophilic tea polyphenols, Epi gallocatechin-3-O-gallate, Epigallocatechin-3-O-gallate-4'-O-hexadecanate, Isolation, High-speed countercurrent chromatography

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