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Journal of Zhejiang University SCIENCE A
ISSN 1673-565X(Print), 1862-1775(Online), Monthly
2004 Vol.5 No.12 P.1608-1612
Characterization of cellulose acetate micropore membrane immobilized acylase I
Abstract: This paper describes an innovative method for the immobilization of acylase I, which was entrapped into the CA-CTA micropore membrane. The most suitable casting solutions proportion for immobilizing the enzyme was obtained through orthogonal experiment. Properties of the enzyme membrane were investigated and compared with those of free enzyme and blank membrane. The thermal stability and pH stability of the enzyme inside the membrane were changed by immobilization. The optimum pH was found to be 6.0, which changes 1.0 unit compared with that of free acylase I. The optimum temperature was found to be about 90 °C, which is higher than that of free acylase I (60 °C). Experimental results showed that immobilization had effects on the kinetic parameters of acylase I.
Key words: Acylase I, CA-CTA micropore membrane, Enzyme immobilization, Orthogonal experiment
References:
Open peer comments: Debate/Discuss/Question/Opinion
<1>
vishal@No address<vishalgaikwad400@gmail.com>
2013-07-16 14:41:44
Ill like to read this article because im interested on the enzyme immobilization methods.
2010-05-28 20:23:36
i would like to know whether immobilization brought about any change in the catalytic activity of the enzyme
Hiram@UAdeC<Orionorbital@hotmail.com>
2010-05-25 10:07:10
Ill like to read this article, because im interested on the enzyme immobilization methods.
DOI:
10.1631/jzus.2004.1608
CLC number:
Q55
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Received:
2003-11-21
Revision Accepted:
2004-07-17
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