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Journal of Zhejiang University SCIENCE B

ISSN 1673-1581(Print), 1862-1783(Online), Monthly

2005 Vol.6 No.1 P.11-13

Construction of a eukaryotic expression plasmid of Humanin

LUO Ben-yan, CHEN Xiang-ming, TANG Min, CHEN Feng, CHEN Zhi

Department of Neurology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; Department of Anesthesiology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; Institute of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

lobenyan@263.net

Abstract: Objective: To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin. Methods: The recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Humanin gene fragments, about 100 bp length, were obtained. Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing. Results: Recombinant plasmid DNA successfully produced a band which had the same size as that of the Humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence. Conclusions: A eukaryotic expression plasmid of Humanin was successfully constructed.

Key words: Humanin, Alzheimer's disease, Eukaryotic expression


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DOI:

10.1631/jzus.2005.B0011

CLC number:

R74

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Received:

2004-07-14

Revision Accepted:

2004-09-30

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