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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2005 Vol.6 No.6 P.454-456
Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii
Abstract: RNA methyltransferase is responsible for transferring methyl and resulting in methylation on the bases or ribose ring of RNA, which existed widely but mostly remains an open question. A recombinant protein PH1948 predicting RNA methyltransferase from Pyrococcus horikoshii OT3 has been crystallized. The crystals of selenomethionyl PH1948 belong to space group C2, with unit-cell parameters a=207.0 Å, b=43.1 Å, c=118.2 Å, β=92.1°, and diffract X-rays to 2.2 Å resolution. The VM value was determined to be 2.8 Å3/Da, indicating the presence of four protein molecules in the asymmetric unit.
Key words: Pyrococcus horikoshii, Methyltransferase, X-ray diffraction, Protein crystallography
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DOI:
10.1631/jzus.2005.B0454
CLC number:
Q5
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Received:
2005-03-25
Revision Accepted:
2005-04-10
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