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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2005 Vol.6 No.11 P.1063-1068
Dependence of the E. coli promoter strength and physical parameters upon the nucleotide sequence
Abstract: The energy of interaction between complementary nucleotides in promoter sequences of E. coli was calculated and visualized. The graphic method for presentation of energy properties of promoter sequences was elaborated on. Data obtained indicated that energy distribution through the length of promoter sequence results in picture with minima at –35, –8 and +7 regions corresponding to areas with elevated AT (adenine-thymine) content. The most important difference from the random sequences area is related to –8. Four promoter groups and their energy properties were revealed. The promoters with minimal and maximal energy of interaction between complementary nucleotides have low strengths, the strongest promoters correspond to promoter clusters characterized by intermediate energy values.
Key words: DNA sequence, Promoter strength, Nucleotide pair energy, −35 sequence, −10 sequence, +7 sequence
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<1>
kannadasan@Manonmaniam Sudaranar University<Kannagenes@gmail.com>
2013-04-28 14:13:31
Hi My Name is Kannadasan Working in small biotech company as Scientific Assistant. Im looking for E. coli promoters. I found your page. Hope I will be helped by this Paper.
DOI:
10.1631/jzus.2005.B1063
CLC number:
Q78
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Received:
2005-07-07
Revision Accepted:
2005-09-07
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