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Journal of Zhejiang University SCIENCE A

ISSN 1673-565X(Print), 1862-1775(Online), Monthly

2007 Vol.8 No.9 P.1514-1520

Reuse of waste frying oil for production of rhamnolipids using Pseudomonas aeruginosa zju.u1M

ZHU Yong, GAN Jun-jiang, ZHANG Guo-liang, YAO Bin, ZHU Wen-jie, MENG Qin

Shool of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China; School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310032, China; Institute of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China

mengq@zju.edu.cn

Abstract: In this work, rhamnolipid production was investigated using waste frying oil as the sole carbon source. By culture in shaking flasks, a naturally isolated strain synthesized rhamnolipid at concentration of 12.47 g/L and its mutant after treatment by UV light increased this productivity to 24.61 g/L. Fermentation was also conducted in a 50 L bioreactor and the productivity reached over 20 g/L. Hence, with a stable and high productive mutant strain, it could be feasible to reuse waste frying oil for rhamnolipid production on industrial scale.

Key words: Rhamnolipid, Pseudomonas aeruginosa, Biosurfactant, Waste frying oil


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DOI:

10.1631/jzus.2007.A1514

CLC number:

Q939.9

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Received:

2006-04-20

Revision Accepted:

2007-02-21

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