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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2012 Vol.13 No.1 P.20-28
Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro
Abstract: Objective: To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs). Methods: MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5–20 ng/ml. Proliferation of MSCs was determined as the mean doubling time. Expression of CXC chemokine receptor 4 (CXCR4) and migration property were determined by flow cytometry and transwell migration essay, respectively. mRNA expression of GATA-4 and collagen II was determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: The mean doubling time of MSC proliferation was decreased, and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1, all in a dose-dependent manner, while the optimal concentration of IGF-1 on proliferation and migration was different. IGF-1 did not affect the expression of GATA-4 or collagen II mRNA. Conclusions: IGF-1 dose-dependently stimulated the proliferation of MSCs, upregulated the expression of CXCR4, and accelerated migration. There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone.
Key words: Mesenchymal stem cells (MSCs), Proliferation, Differentiation, Insulin-like growth factor-1 (IGF-1), CXC chemokine receptor 4 (CXCR4), Migration
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Open peer comments: Debate/Discuss/Question/Opinion
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DOI:
10.1631/jzus.B1100117
CLC number:
Q291; R542.2
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On-line Access:
2011-12-30
Received:
2011-04-09
Revision Accepted:
2011-08-30
Crosschecked:
2011-12-10