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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2014 Vol.15 No.3 P.295-302
Anti-adipogenic effects of extracts of Ficus deltoidea var. deltoidea and var. angustifolia on 3T3-L1 adipocytes
Abstract: Objective: This study examined the anti-adipogenic effects of extracts of Ficus deltoidea var. deltoidia and var. angustifolia, a natural slimming aid, on 3T3-L1 adipocytes. Methods: Methanol and water extracts of leaves of the F. deltoidea varieties were analyzed to determine their total flavonoid content (TFC) and total phenolic content (TPC), respectively. The study was initiated by determining the maximum non-toxic dose (MNTD) of the methanol and water extracts for 3T3-L1 preadipocytes. Possible anti-adipogenic effects were then examined by treating 2-d post confluent 3T3-L1 preadipocytes with either methanol extract or water extract at MNTD and half MNTD (½MNTD), after which the preadipocytces were induced to form mature adipocytes. Visualisation and quantification of lipid content in mature adipocytes were carried out through oil red O staining and measurement of optical density (OD) at 520 nm, respectively. Results: The TFCs of the methanol extracts were 1.36 and 1.97 g quercetin equivalents (QE)/100 g dry weight (DW), while the TPCs of the water extracts were 5.61 and 2.73 g gallic acid equivalents (GAE)/100 g DW for var. deltoidea and var. angustilofia, respectively. The MNTDs determined for methanol and water extracts were (300.0±28.3) and (225.0±21.2) µg/ml, respectively, for var. deltoidea, while much lower MNTDs [(60.0±2.0) µg/ml for methanol extracts and (8.0±1.0) µg/ml for water extracts] were recorded for var. angustifolia. Studies revealed that the methanol extracts of both varieties and the water extracts of var. angustifolia at either MNTD or ½MNTD significantly inhibited the maturation of preadipocytes. Conclusions: The inhibition of the formation of mature adipocytes indicated that leaf extracts of F. deltoidea could have potential anti-obesity effects.
Key words: Adipogenesis, Ficus deltoidea, Flavonoids, Natural products, Obesity
研究方法：用甲醇和水分别对两个品种金钱榕的叶片进行提取，并分别分析了提取物中的总黄酮含量（TFC）和总酚含量（TPC）。通过MTT法确定提取物对3T3-L1脂肪细胞的最大无毒剂量（MNTD）。然后，通过油红O染色和吸光度测定来定性和定量研究提取物对抗脂肪细胞脂肪沉积的作用。
重要结论：金钱榕两个品种deltoidea和angustifolia的甲醇提取物中的TFC含量分别为1.36和1.97 g QE/100 g DW，水提取物中的TPC含量分别为5.61和2.73 g GAE/100 g DW。金钱榕deltoidea品种的甲醇和水提取物对3T3-L1细胞的MNTD分别为（300.0±28.3）和（225.0±21.2）µg/ml，而angustifolia品种的MNTDs则较低（其甲醇和水提取物的MNTDs分别为（60.0±2.0）和（8.0±1.0）μg/ml）。金钱榕两个品种的甲醇提取物和angustifolia品种的水提取物在MNTD或½MNTD的浓度下，均具有显著抑制前脂肪细胞的成熟的作用。因此，根据其抑制成熟脂肪细胞形成的结果可以得出金钱榕叶片提取物具有潜在的抗肥胖效果。
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DOI:
10.1631/jzus.B1300123
CLC number:
R285
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On-line Access:
2014-03-04
Received:
2013-04-25
Revision Accepted:
2013-10-14
Crosschecked:
2014-02-21