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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2014 Vol.15 No.1 P.100-108
Molecular identification of Sporothrix clinical isolates in China
Abstract: In this study, we investigated the molecular phylogeny of 64 clinical isolates which were identified as Sporothrix schenckii sensu lato by morphological identification. All of the strains were isolates from patients from several provinces in China. The phylogeny was inferred by DNA sequence analyses based on datasets of the ribosomal internal transcribed spacer (ITS) and a combined ITS and partial β-tubulin region. Reference sequences were retrieved from GenBank. Results showed that all of the isolates were clustered in a distinct clade with a type of Sporothrix globosa. Our analysis showed that S. globosa is the causal agent of the tested sporotrichosis in China, rather than S. schenckii that was generally believed to be the case. The existence of S. schenckii in China remains to be confirmed. This study improved our understanding of the distribution of the species in S. schenckii complex.
Key words: Internal transcribed spacer (ITS), Diagnosis, Sporothrix globosa, Taxonomy, Phylogeny
创新方法:构建中国地区临床孢子丝菌系统进化树,用分子生物学方法对致病菌种进行重新鉴定。
研究手段:多基因测序和系统发育分析。
重要结论:球形孢子丝菌可能为中国地区孢子丝菌病的主要病原菌,申克孢子丝菌在中国地区是否存在尚需进一步验证。
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DOI:
10.1631/jzus.B1300136
CLC number:
R75
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On-line Access:
2024-08-27
Received:
2023-10-17
Revision Accepted:
2024-05-08
Crosschecked:
2013-12-27