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Journal of Zhejiang University SCIENCE B

ISSN 1673-1581(Print), 1862-1783(Online), Monthly

Phenolic contents and antioxidant capacities of Thai-Makham Pom (Phyllanthus emblica L.) aqueous extracts

Abstract: This study was aimed at determining the amount of the total phenolic contents (TPCs) and to evaluate the antioxidant activities of different extracts (leaves, branches, and barks) of Phyllanthus emblica (PE) L., a traditional medicinal plant that has been recently used as an anti-inflammatory, wound healing, and anti-aging agent. PE leaves, branches, or barks were aqueous-extracted and the amount of the TPC was determined using a Folin-Ciocalteu reagent method. The antioxidant activity of each PE extract was as-sessed using 1,1-diphenyl-2-picrylhydrazyl radical scavenging and ferric reducing antioxidant power (FRAP) methods. Among plant extracts, the PE bark extract possessed the highest TPC. The TPCs of the PE leaves, branches, and barks were (513.83±20.52), (650.50±9.76), and (2196.33±11.02) mg gallic acid equivalent/g dried material, respectively. Interest-ingly, the IC50, which was calculated from 50% inhibition against concentration (µg/ml) plot, of the PE leaf ((7.72±0.25) µg/ml), branch ((6.92±0.22) µg/ml), or bark ((6.54±0.27) µg/ml) extract was lower than that of the ascorbic acid ((8.06±0.01) µg/ml). As calibrated to the standard, the potent reducing power abilities of the PE leaf, branch, and bark extracts were (696.73±78.48), (729.33±36.9), and (966±64.73) mg/g ascorbic acid equivalent, respectively.

Key words: Total phenolic content, Antioxidant activity, Phyllanthus emblica L., Traditional medicinal plant

Chinese Summary  <33> 泰国余甘子水提物中总酚含量及抗氧化活性

本文概要:通过对余甘子不同部位(叶、枝和树皮)水提物的总酚含量的测定及体外抗氧化活性的研究,为其体内及临床试验研究奠定了基础。余甘子叶、枝和树皮的水提物皆具有抗氧化活性,其中树皮中的总酚含量最高,抗氧化活性最强。

关键词组:余甘子;总酚含量;抗氧化能力;传统药用植物


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DOI:

10.1631/jzus.B1300284

CLC number:

Q505; R931.71

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On-line Access:

2014-04-06

Received:

2013-11-10

Revision Accepted:

2014-02-10

Crosschecked:

2014-03-10

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