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Journal of Zhejiang University SCIENCE B

ISSN 1673-1581(Print), 1862-1783(Online), Monthly

2016 Vol.17 No.6 P.447-454

Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens

Hong-yun Peng, Xue-hong Zhang, Jin-zhong Xu

Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou 310058, China; Institution of Marine Biology, Ocean College, Zhejiang University, Hangzhou 310058, China

penghongyun@zju.edu.cn

Abstract: Elsholtzia splendens (Lamiaceae) is a copper-tolerant plant species growing on copper deposits in the south of China. Chromatographic separation of n-BuOH extracts from the flowering aerial biomass afforded apigenin-7-O-β-D-glycoside, using macroporous resin, Sephadex™ LH-20 gel, polyamide resin as well as preparative high-performance liquid chromatography (P-HPLC) columns. Chemical structure was elucidated using HPLC/ESI-MS (electrospray ionization-mass spectrometry), Fourier transform infrared (FTIR), and 1D- and 2D-nuclear magnetic resonance (NMR). Apigenin-7-O-β-D-glycoside could be the post-harvesting product from E. splendens biomass.

Key words: Chromatographic separation, Copper-tolerant plant, Elsholtzia splendens, Flavonoid glycoside, Structure elucidation

Chinese Summary  <30> 从铜耐性植物海州香薷中提取分离芹菜素糖苷

目的：建立从铜耐性植物海州香薷地上部提取分离芹菜素糖苷的工艺技术，探索铜耐性植物海州香薷资源化途径。
创新点：结合使用大孔树脂、凝胶树脂、聚酰胺树脂多种色谱柱技术，从铜耐性植物海州香薷中提取分离纯度达98%的黄酮苷类化合物芹菜素糖苷。
方法：以铜耐性植物海州香薷为原料，用60%乙醇溶液浸提，提取液依次使用石油醚、乙酸乙酯、饱和正丁醇萃取。实验中，对饱和正丁醇萃取层，使用D101型大孔吸附树脂粗分离提取，Sephadex™ LH-20型凝胶筛分分离，并借助半制备液相和聚酰胺树脂分离纯化，得到芹菜素糖苷单体（图1）。利用紫外光谱、液相色谱–质谱联用、红外光谱、一维和二维核磁图谱，鉴定芹菜素糖苷结构（图2~5）。
结论：芹菜素糖苷是铜耐性植物海州香薷体内的天然产物，利用色谱技术可以从海州香薷植物体中分离出芹菜素糖苷单体。

关键词组：色谱分离；铜耐性植物；海州香薷；黄酮苷；结构解析


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DOI:

10.1631/jzus.B1500242

CLC number:

X173; X53

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On-line Access:

2024-08-27

Received:

2023-10-17

Revision Accepted:

2024-05-08

Crosschecked:

2016-05-12

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