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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2016 Vol.17 No.11 P.864-873
In vitro remineralization of hybrid layers using biomimetic analogs
Abstract: Resin-dentin bond degradation is a major cause of restoration failures. The major aim of the current study was to evaluate the impact of a remineralization medium on collagen matrices of hybrid layers of three different adhesive resins using nanotechnology methods. Coronal dentin surfaces were prepared from freshly extracted premolars and bonded to composite resin using three adhesive resins (FluoroBond II, Xeno-III-Bond, and iBond). From each tooth, two central slabs were selected for the study. The slabs used as controls were immersed in a simulated body fluid (SBF). The experimental slabs were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs (biomineralization medium (BRM)). Eight slabs per group were retrieved after 1, 2, 3, and 4 months, respectively and immersed in Rhodamine B for 24 h. Confocal laser scanning microscopy was used to evaluate the permeability of hybrid layers to Rhodamine B. Data were analyzed by analysis of variance (ANOVA) and Tukey’s honest significant difference (HSD) tests. After four months, all BRM specimens exhibited a significantly smaller fluorescent area than SBF specimens, indicating a remineralization of the hybrid layer (P≤0.05). A clinically applicable biomimetic remineralization delivery system could potentially slow down bond degradation.
Key words: Remineralization, Dentin, Adhesive resin, Biomimetic analog, Altered collagen, Confocal laser scanning microscopy (CLSM), Fluorescence
创新点：（1）多数仿生再矿化研究集中于通过透射电子显微镜观察牙本质粘结剂界面的再矿化情况，鲜有采用激光共聚焦显微镜（CLSM）作为观察手段的。虽有研究应用CLSM，但也仅是从定性的角度比较再矿化效应，没有定量分析比较，难以令人信服。而本项研究从定性和定量两方面分析对比。（2）本研究从当前牙本质粘结剂系统（共七代）常用的后四代中选择典型代表产品为研究对象，便于横向比较各类粘结剂。（3）本研究的定量结果除了比较同一时间实验组和对照组的矿化情况，还增加了纵向分析思路，进一步比较矿化程度随时间的变化以及不同牙本质粘结剂粘结界面的矿化情况。
方法：将96颗健康前磨牙按照FluoroBond II、Xeno-III和iBond粘结剂随机分为3组，每颗牙均暴露表层牙本质。3种粘结剂分别严格按照各自产品说明书处理牙本质表面，牙合面堆制5 mm厚的树脂。每颗牙沿牙合-龈向切成0.9 mm厚的薄片，获取中间两片样本用于矿化组和模拟组。矿化组采用含聚乙烯磷酸和聚丙烯酸的再矿化液浸泡；模拟组采用不含聚乙烯磷酸和聚丙烯酸的模拟体液浸泡。各组标本在储存1、2、3和4个月后，各取出8片，经苏丹明B荧光染料染色24 h，冲洗，吹干，置CLSM下观察渗入混合层及粘结层的荧光情况，测量荧光面积、平均荧光量及总荧光量。所有数据统计方法采用方差分析（ANOVA）和Tukey’s HSD检验分析。
结论：本研究中聚丙烯酸和聚乙烯磷酸双仿生类似物分子对FluoroBond II、Xeno-III及iBond粘结剂均显示不同程度的再矿化效应，其中对iBond粘结剂再矿化效应最明显，FluoroBond II粘结剂次之，Xeno-III粘结剂再矿化效应较差，但能够起到抵制基底矿物继续丢失或阻止胶原降解的作用。CLSM结合应用苏丹明B是量化混合层再矿化的一项有效手段。因此，上述双仿生类似物分子应用于口腔粘结剂修复材料促使混合层再矿化，具有良好的应用前景，值得进一步深入研究。
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DOI:
10.1631/jzus.B1600151
CLC number:
R783.1
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On-line Access:
2016-11-03
Received:
2016-04-05
Revision Accepted:
2016-06-23
Crosschecked:
2016-10-18