|
|
Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2017 Vol.18 No.9 P.797-806
Antioxidant activity of alcohol aqueous extracts of Crypthecodinium cohnii and Schizochytrium sp.
Abstract: Crypthecodinium cohnii (dinoflagellate) and Schizochytrium sp. (thraustochytrid) are the main sources for docosahexaenoic acid (DHA). The present study aimed to evaluate the antioxidant activity of petroleum ether, ethyl acetate, n-butanol, and water fractions of alcohol aqueous extracts of these two microalgae and to provide a theoretical basis for comprehensive utilization. The antioxidant activity was determined by total antioxidant capacity (TAC) determination, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, ferrous ion-chelating ability (FICA) assay, and reducing power (RP) assay. The total phenolic content (TPC) and total flavonoid content (TFC) were also measured by the Folin-Ciocalteu and spectrophotometry methods, respectively. The results indicated that the extracts from these two microalgae possessed good antioxidant capacity. Analysis showed that most antioxidant performance indicators (TAC, DPPH, and RP) were positively correlated with the TPC of the extracts, suggesting that the phenolics might be the major components in C. cohnii and Schizochytrium sp., contributing to their antioxidative function. Therefore, the polar fractions of C. cohnii and Schizochytrium sp. could be further examined and considered for application in health products or cosmetics.
Key words: Crypthecodinium cohnii; Schizochytrium sp.; Alcohol aqueous extract; Antioxidant activity; Total phenolics
创新点：隐甲藻和裂殖壶藻是二十二碳六烯酸（DHA）的重要原料，然而提取DHA后剩余的藻渣未得到充分利用。本文首次对两种微藻的极性提取物的不同极性部位进行抗氧化活性评价，并初步确定多酚类化合物是其发挥抗氧化作用的主要物质成分，为综合利用两种微藻提供了理论依据和参考。
方法：用70%乙醇浸提隐甲藻和裂殖壶藻藻粉，所得粗提物分别使用石油醚、乙酸乙酯、正丁醇和水依次萃取，得到不同极性组分萃取物。采用总抗氧化能力、2,2-二苯基-1-三硝基苯肼（DPPH）自由基清除能力、亚铁离子螯合能力及总还原力等方法对不同极性组分的萃取物进行抗氧化活性评价。采用Folin-Ciocalteu方法测定样品中总多酚含量，采用分光光度法测定样品中总黄酮含量。
结论：本实验结果显示，隐甲藻和裂殖壶藻醇水提物的不同极性组分具有较好的抗氧化作用（图2~5）。相关性分析结果表明萃取物中的多酚类化合物与其抗氧化水平显著相关（表2和表3），因此，我们推测多酚类化合物是两种微藻的主要抗氧化成分。综上所述，隐甲藻和裂殖壶藻具有综合开发利用潜力，可深入研究。
关键词组：
References:
Open peer comments: Debate/Discuss/Question/Opinion
<1>
Jin-hui Yu@a. Institute for Genomic Engineered Animal Models of Human Diseases, Dalian Medical University, Dalian, Liaoning, 116044, China; b. Shandong Center of Crop Germplasm Resources, Jinan, Shandong, 250100, China; d. College of Integrative Medicine, Dalian Med<faith_2002@163.com>
2016-11-14 11:16:42
Dear editor, I found some problems as followed:
(1) The affiliation of each author and corresponding authors were not labeled.
(2) yingjiewu@dlmedu.edu.cn should be changed to yingjiewu@dmu.edu.cn.
Thank you!
Jinhui Yu
DOI:
10.1631/jzus.B1600367
CLC number:
Q939.9
Download Full Text:
Downloaded:
3608
Download summary:
<Click Here>Downloaded:
1729Clicked:
5439
Cited:
0
On-line Access:
2017-09-05
Received:
2016-08-19
Revision Accepted:
2016-10-31
Crosschecked:
2017-08-17