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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2021 Vol.22 No.9 P.767-773
Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628
Abstract: Streptomyces are famous for their ability to synthesize a large number of bioactive compounds as secondary metabolites containing antibiotics, enzyme inhibitors, and other small molecules with potential physiological activity (Niu et al., 2016; Song et al., 2019; Yin et al., 2019). Secondary metabolites are produced by a multi-step reaction of a primary metabolite as a precursor (Liu et al., 2013; Li et al., 2021). Therefore, it is of great research significance to increase the overall synthesis level of antibiotics by increasing the amount of synthesis of precursors.
Key words: S. diastatochromogenes 1628; SAM; MetK; Toyocamycin; Tetraene macrolides
创新点：SAM是甲基的重要供体，可为次级代谢产物提供前体，同时也可以作为信号增强次级代谢产物合成基因的表达。因此，本研究首次探究了SAM与S. diastatochromogenes1628次级代谢产物合成的相关性。
方法：以S. diastatochromogenes1628为对象，通过体外添加SAM以及在S. diastatochromogenes1628体内过表达、敲除和回补SAM编码基因metKsd，考察S. diastatochromogenes1628次级代谢产物产量的变化。
结论：由于渗透性差，外源添加SAM对S. diastatochromogenes1628合成次级代谢产物（丰加霉素和三种四烯大环内酯类抗生素：四霉素A、四霉素P和四烯菌素B）没有影响；敲除、回补和过表达基因metKsd证实增加胞内SAM可提高S. diastatochromogenes1628三种四烯大环内酯类抗生素的产量，且可促进四烯大环内酯类化合物合成关键基因的表达，而胞内SAM浓度的变化对丰加霉素的产量和及其关键基因的表达均无明显影响。
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DOI:
10.1631/jzus.B2100115
CLC number:
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On-line Access:
2024-08-27
Received:
2023-10-17
Revision Accepted:
2024-05-08
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