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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2023 Vol.24 No.7 P.617-631
Scutellarin prevents acute alcohol-induced liver injury via inhibiting oxidative stress by regulating the Nrf2/HO-1 pathway and inhibiting inflammation by regulating the AKT, p38 MAPK/NF-κB pathways
Abstract: Alcoholic liver disease (ALD) is the most frequent liver disease worldwide, resulting in severe harm to personal health and posing a serious burden to public health. Based on the reported antioxidant and anti-inflammatory capacities of scutellarin (SCU), this study investigated its protective role in male BALB/c mice with acute alcoholic liver injury after oral administration (10, 25, and 50 mg/kg). The results indicated that SCU could lessen serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and improve the histopathological changes in acute alcoholic liver; it reduced alcohol-induced malondialdehyde (MDA) content and increased glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) activity. Furthermore, SCU decreased tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), andIL-1β messenger RNA (mRNA) expression levels, weakened inducible nitric oxide synthase (iNOS) activity, and inhibited nucleotide-binding oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome activation. Mechanistically, SCU suppressed cytochrome P450 family 2 subfamily E member 1 (CYP2E1) upregulation triggered by alcohol, increased the expression of oxidative stress-related nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) pathways, and suppressed the inflammation-related degradation of inhibitor of nuclear factor-κB (NF-κB)-α (IκBα) as well as activation of NF-κB by mediating the protein kinase B (AKT) and p38 mitogen-activated protein kinase (MAPK) pathways. These findings demonstrate that SCU protects against acute alcoholic liver injury via inhibiting oxidative stress by regulating the Nrf2/HO-1 pathway and suppressing inflammation by regulating the AKT, p38 MAPK/NF-κB pathways.
Key words: Scutellarin; Oxidative stress; Alcoholic liver disease; Inflammation
机构：1中国计量大学，质量与安全工程学院，中国杭州，310018；2中国计量大学，机电工程学院，中国杭州，310018；3浙江工业大学，机械工程学院，中国杭州，310023
目的：研究丁腈橡胶硬度对O形液压杆封静/动态密封性能的影响，为其工程中橡胶硬度的选型提供理论参考。
创新点：1.基于混合弹流润滑模型，分析了丁腈橡胶硬度对O形液压杆封静/动态密封性能的影响；2.以低泄漏、低摩擦为目标，获得了静/动态应用下丁腈橡胶O形液压杆封的橡胶硬度优选范围。
方法：1.通过数值分析静接触压力、von Mises应力、最小液膜厚度、泄漏率和摩擦力，揭示丁腈橡胶硬度对O形液压杆封静/动态密封性能的影响机理；2.以最大静接触压力和最大von Mises应力作为静密封性能评价指标，以及以净泄漏量和摩擦功耗作为动密封性能的评价指标，确定丁腈橡胶O形液压杆封的最佳橡胶硬度值。
结论：1.随着橡胶硬度的增加，丁腈橡胶O形液压杆封的抗变形能力增强，静接触压力增大，最小液膜厚度减小，泄漏量减小，摩擦力增大；2.低硬度密封易产生挤出效应和应力集中现象，因此更适合在高速或低密封性要求的场合使用；3.高硬度密封具有更优的静/动态密封性能，所以特别适用于高压工况场合，但由于其摩擦力较大，因此在满足泄漏要求的前提下应尽量选择相对较低的橡胶硬度以减小摩擦。
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DOI:
10.1631/jzus.B2200612
CLC number:
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On-line Access:
2023-07-15
Received:
2022-11-29
Revision Accepted:
2023-01-03
Crosschecked:
2023-07-17