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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2024 Vol.25 No.9 P.789-795
Unveiling the innovative green synthesis mechanism of selenium nanoparticles by exploiting intracellular protein elongation factor Tu from Bacillus paramycoides
Abstract: Selenium nanoparticles (SeNPs) have garnered extensive research interest and shown promising applications across diverse fields owing to their distinctive properties, including antioxidant, anticancer, and antibacterial activity (Ojeda et al., 2020; Qu et al., 2023; Zambonino et al., 2021, 2023). Among the various approaches employed for SeNP synthesis, green synthesis has emerged as a noteworthy and eco-friendly methodology. Keshtmand et al. (2023) underscored the significance of green-synthesized SeNPs, presenting a compelling avenue in this domain. This innovative strategy harnesses the potential of natural resources, such as plant extracts or microorganisms, to facilitate the production of SeNPs.
Key words: Selenium nanoparticles (SeNPs); Bacillus paramycoides; Elongation Factor Tu (EF-Tu); Selenite reduction; Green synthesis mechanism
1淮阴工学院生命科学与食品工程学院， 中国淮安市， 223003
2淮阴工学院江苏省益生菌制剂重点建设实验室， 中国淮安市， 223003
摘要：纳米硒（SeNPs）因具有独特特性而备受关注，在各领域均有较好的应用前景。因此，开发高效、环保的SeNPs合成方法具有重要意义。本实验室保存的Bacillus paramycoides 24522在24 h内可将亚硒酸盐还原为具有高稳定性和高分散性的SeNPs，其平均直径为150 nm（100~200 nm），zeta电位为?29.9 eV。本研究提出了一种绿色新颖的还原机制，即利用B. paramycoides 24522中细胞质蛋白延伸因子Tu（EF-Tu）还原合成SeNPs，并经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和液相色谱串联质谱（LC-MS/MS）分析，鉴定EF-Tu分子质量约为43 kDa，含395个氨基酸。实时荧光定量聚合酶链反应（qPCR）结果显示EF-Tu的信使RNA（mRNA）表达量增加8.9倍，这进一步证实了EF-Tu参与了亚硒酸盐的还原。透射电镜（TEM）成像结果显示，SeNPs的合成发生在细胞内。综上可知，小SeNPs最初在细胞内作为种子与EF-Tu相互作用，随后经历成熟过程，并最终通过细胞裂解释放到培养基中。这种创新的绿色合成机制为SeNPs的生产提供了宽广的前景，有助于推进环境友好型纳米材料的合成。
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DOI:
10.1631/jzus.B2300738
CLC number:
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On-line Access:
2024-08-27
Received:
2023-10-17
Revision Accepted:
2024-05-08
Crosschecked:
2024-09-23