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Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly
2025 Vol.26 No.2 P.185-199
Effects of glyphosate, antibiotics, and an anticoccidial drug on pancreatic gene expression and blood physiology in broilers
Abstract: Drugs and pesticide residues in broiler feed can compromise the therapeutic and production benefits of antibiotic (ANT) application and affect gene expression. In this study, we analyzed the expression of 13 key pancreatic genes and blood physiology parameters after administering one maximum residue limit of herbicide glyphosate (GLY), two ANTs, and one anticoccidial drug (AD). A total of 260 Ross 308 broilers aged 1–40 d were divided into the following four groups of 65 birds each: control group, which was fed the main diet (MD), and three experimental groups, which were fed MD supplemented with GLY, GLY+ANTs (enrofloxacin and colistin methanesulfonate), and GLY+AD (ammonium maduramicin), respectively. The results showed that the addition of GLY, GLY+ANTs, and GLY+AD caused significant changes in the expression of several genes of physiological and economic importance. In particular, genes related to inflammation and apoptosis (interleukin 6 (IL6), prostaglandin-endoperoxide synthase 2 (PTGS2), and caspase 6 (CASP6)) were downregulated by up to 99.1%, and those related to antioxidant protection (catalase (CAT), superoxide dismutase 1 (SOD1) and peroxiredoxin 6 (PRDX6)) by up to 98.6%, compared to controls. There was also a significant decline in the values of immunological characteristics in the blood serum observed in the experimental groups, and certain changes in gene expression were concordant with changes in the functioning of the pancreas and blood. The changes revealed in gene expression and blood indices in response to GLY, ANTs, and AD provide insights into the possible mechanisms of action of these agents at the molecular level. Specifically, these changes may be indicative of physiological mechanisms to overcome the negative effects of GLY, GLY+ANTs, and GLY+AD in broilers.
Key words: Glyphosate; Antibiotic; Anticoccidial drug; Pancreas; Broiler; Gene expression; Blood parameter
1Molecular Genetics and Microbiomics Laboratory, BIOTROF+ Ltd., Pushkin, St. Petersburg 196602, Russia
2Federal State Budgetary Educational Institution of Higher Education "St. Petersburg State Agrarian University," Pushkin, St. Petersburg 196605, Russia
3School of Natural Sciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK
4Animal Genomics and Bioresource Research Unit (AGB Research Unit), Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand
5L. K. Ernst Federal Research Center for Animal Husbandry, Dubrovitsy, Podolsk, Moscow Oblast 142132, Russia
摘要：肉鸡饲料中的药物和农药残留可能会影响抗生素（ANT）的治疗和生产效益，进而影响基因表达。在本研究中，我们分析了在施用最大残留限量的除草剂草甘膦（GLY）、两种ANT和一种抗球虫药物（AD）后，13个关键胰腺基因的表达和血液生理参数的变化。将260只1~40日龄的Ross 308肉鸡分为以下四组，每组65只：（1）对照组，饲喂基础日粮（MD）；（2）GLY组，饲喂添加GLY的MD；（3）GLY+ANTs组，饲喂添加GLY和ANT的MD；（4）GLY+AD组，饲喂添加GLY和AD的MD。结果显示，添加GLY、GLY+ANTs和GLY+AD会导致多个具有生理和经济意义的基因表达发生显著变化。特别是，与炎症和凋亡相关的基因（如白细胞介素6（IL6）、前列腺素内过氧化物合酶2（PTGS2）和半胱天冬酶6（CASP6））的表达下调高达99.1%，而与抗氧化保护相关的基因（如过氧化氢酶（CAT）、超氧化物歧化酶1（SOD1）和过氧化还原酶6（PRDX6））的表达下调高达98.6%。实验组中还观察到血清免疫学特性值显著下降，某些基因表达的变化与胰腺功能和血液变化一致。GLY、ANT和AD引起的基因表达和血液指标变化揭示了这些物质在分子水平上的可能作用机制，同时也表明肉鸡克服GLY、GLY+ANT和GLY+AD负面影响的生理机制。
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DOI:
10.1631/jzus.B2300767
CLC number:
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On-line Access:
2025-02-26
Received:
2023-10-25
Revision Accepted:
2024-02-07
Crosschecked:
2025-02-27