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Abstract: The cell cycle consists of four distinct phases: G0/G1, S (DNA synthesis), G2, and M (mitosis). The G1 to S transition is typified by an accumulation of 4',‍6-diamidino-2-phenylindole (DAPI) signal that indicates the rapid DNA synthesis initiated at special sites on DNA, generally called the Ori (origin of replication) (Alfa et al., 1989). The cell division cycle 10 (Cdc10)‍-dependent transcript 1 (Cdt1) is bestowed the term “replication origin licensing factor” for its role in warranting replication once (and only once) per round of the eukaryotic cell cycle, during the S phase (Pozo and Cook, 2017). In a normal cell cycle, Cdt1 is present only in the G1 and S entry phases, whereas Geminin, a protein that targets Cdt1 for S-phase-dependent proteolysis, is present in the S and G2 phases. Failure of this gatekeeping task as observed in cdt1 overexpression, for example in yeast, leads to inappropriate origin firing (also termed re-replication (Vaziri et al., 2003)) and eventually DNA damage checkpoint activation (Kanellou et al., 2020).

斑马鱼cdt1缺失导致类似人类Meier-Gorlin疾病的侏儒症及性腺发育不全综合征

[1]AlfaCE, BooherR, BeachD, et al., 1989. Fission yeast cyclin: subcellular localisation and cell cycle regulation. J Cell Sci, 1989(S12):9-19.

[2]BicknellLS, WalkerS, KlingseisenA, et al., 2011. Mutations in ORC1, encoding the largest subunit of the origin recognition complex, cause microcephalic primordial dwarfism resembling Meier-Gorlin syndrome. Nat Genet, 43(4):350-355.

[3]de MunnikSA, HoefslootEH, RoukemaJ, et al., 2015. Meier-Gorlin syndrome. Orphanet J Rare Dis, 10:114.

[4]HofmannJF, BeachD, 1994. cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis. EMBO J, 13‍(2):425-434.

[5]KanellouA, GiakoumakisNN, PanagopoulosA, et al., 2020. The licensing factor Cdt1 links cell cycle progression to the DNA damage response. Anticancer Res, 40(5):2449-2456.

[6]MaZP, ZhuPP, ShiH, et al., 2019. PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components. Nature, 568(7751):259-263.

[7]MaioranoD, MoreauJ, MéchaliM, 2000. XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis. Nature, 404(6778):622-625.

[8]NazarenkoMS, ViakhirevaIV, SkoblovMY, et al., 2022. Meier-Gorlin syndrome: clinical misdiagnosis, genetic testing and functional analysis of ORC6 mutations and the development of a prenatal test. Int J Mol Sci, 23(16):9234.

[9]NishitaniH, LygerouZ, NishimotoT, et al., 2000. The Cdt1 protein is required to license DNA for replication in fission yeast. Nature, 404(6778):625-628.

[10]PozoPN, CookJG, 2017. Regulation and function of Cdt1; a key factor in cell proliferation and genome stability. Genes (Basel), 8(1):2.

[11]VaziriC, SaxenaS, JeonY, et al., 2003. A p53-dependent checkpoint pathway prevents rereplication. Mol Cell, 11(4):997-1008.

[12]WhittakerAJ, RoyzmanI, Orr-WeaverTL, 2000. Drosophila Double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts. Genes Dev, 14(14):1765-1776.

[13]WittkoppN, HuntzingerE, WeilerC, et al., 2009. Nonsense-mediated mRNA decay effectors are essential for zebrafish embryonic development and survival. Mol Cell Biol, 29(13):3517-3528.

[14]WohlschlegelJA, DwyerBT, DharSK, et al., 2000. Inhibition of eukaryotic DNA replication by geminin binding to Cdt1. Science, 290(5500):2309-2312.

[15]YaoLK, ChenJ, WuXT, et al., 2017. Zebrafish cdc6 hypomorphic mutation causes Meier-Gorlin syndrome-like phenotype. Hum Mol Genet, 26(21):4168-4180.