CLC number:
On-line Access: 2020-01-23
Received: 2019-09-28
Revision Accepted: 2020-01-23
Crosschecked: 2020-03-01
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Fu You, Xia Wu, Michael Kelly, Xiongbiao Chen. Bioprinting and in vitro characterization of alginate dialdehyde–gelatin hydrogel bio‑ink[J]. Journal of Zhejiang University Science D, 2020, 3(1): 48-59.
@article{title="Bioprinting and in vitro characterization of alginate
dialdehyde–gelatin hydrogel bio‑ink",
author="Fu You, Xia Wu, Michael Kelly, Xiongbiao Chen",
journal="Journal of Zhejiang University Science D",
volume="3",
number="1",
pages="48-59",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00058-8"
}
%0 Journal Article
%T Bioprinting and in vitro characterization of alginate
dialdehyde–gelatin hydrogel bio‑ink
%A Fu You
%A Xia Wu
%A Michael Kelly
%A Xiongbiao Chen
%J Journal of Zhejiang University SCIENCE D
%V 3
%N 1
%P 48-59
%@ 1869-1951
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00058-8
TY - JOUR
T1 - Bioprinting and in vitro characterization of alginate
dialdehyde–gelatin hydrogel bio‑ink
A1 - Fu You
A1 - Xia Wu
A1 - Michael Kelly
A1 - Xiongbiao Chen
J0 - Journal of Zhejiang University Science D
VL - 3
IS - 1
SP - 48
EP - 59
%@ 1869-1951
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00058-8
Abstract: Cell-laden cardiac patches have recently been emerging to renew cellular sources for myocardial infarction (MI, commonly
know as a heart attack) repair. However, the fabrication of cell-laden patches with porous structure remains challenging due
to the limitations of currently available hydrogels and existing processing techniques. The present study utilized a bioprinting
technique to fabricate hydrogel patches and characterize them in terms of printability, mechanical and biological properties.
Cell-laden hydrogel (or bio-ink) was formulated from alginate dialdehyde (ADA) and gelatin (GEL) to improve the printability, degradability as well as bioactivity. Five groups of hydrogel compositions were designed to investigate the infuence
of the oxidation degree of ADA and hydrogels concentration on the properties of printed scafolds. ADA–GEL hydrogels
have generally shown favorable for living cells (EA.hy926 cells and hybrid human umbilical vein endothelial cell line). The
hydrogel with an oxidation degree of 10% and a concentration ratio of 70/30 (or 10%ADA70–GEL30) demonstrated the
best printability among the groups examined. Formulated hydrogels were also bioprinted with the living cells (EA.hy926),
and the scafolds printed were then subject to the cell culture for 7 days. Our results illustrate that the scafolds bioprinted
from 10%ADA70–GEL30 hydrogels had the best homogenous cell distribution and also the highest cell viability. Taken
together, in the present study we synthesized a newly formulated bio-ink from ADA and GEL and for the fst time, used them
to bioprint cardiac patches, which have the potential to be used in MI repair.
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