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On-line Access: 2021-02-03

Received: 2020-08-19

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Bio-Design and Manufacturing  2021 Vol.4 No.3 P.490-505

http://doi.org/10.1007/s42242-020-00114-3


Peptides-based vaccine against SARS-nCoV-2 antigenic fragmented synthetic epitopes recognized by T cell and ?-cell initiation of specific antibodies to fight the infection


Author(s):  Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, P.O. Box 715, Mecca 21955, Kingdom of Saudi Arabia, Science and Technology Unit, Umm Al-Qura University, P.O. Box 715, Mecca 21955, Kingdom of Saudi Arabia, The Regional Laboratory, Molecular Diagnostics Unit, Department of Molecular Biology, Ministry of Health (MOH), P.O. Box 6251, Mecca, Kingdom of Saudi Arabia, Department of Pathology and Lab Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON K1H 8M5, Canada

Affiliation(s):  School of Automation Science and Electrical Engineering, Beihang University, Beijing 100191, China; more

Corresponding email(s):   zaabduljaleel@uqu.edu.sa, zainulbio@gmail.com

Key Words:  COVID-19, SARS-nCoV-2, Peptide-based vaccine, Cell-penetrating peptides, Lipid membrane, Vaccine efficacy


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Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, P.O. Box 715, Mecca 21955, Kingdom of Saudi Arabia; Science and Technology Unit, Umm Al-Qura University, P.O. Box 715, Mecca 21955, Kingdom of Saudi Arabia; The Regional Laboratory, Molecular Diagnostics Unit, Department of Molecular Biology, Ministry of Health (MOH), P.O. Box 6251, Mecca, Kingdom of Saudi Arabia; Department of Pathology and Lab Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON K1H 8M5, Canada. Peptides-based vaccine against SARS-nCoV-2 antigenic fragmented synthetic epitopes recognized by T cell and ?-cell initiation of specific antibodies to fight the infection[J]. Journal of Zhejiang University Science D, 2021, 4(3): 490-505.

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author="Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, P.O. Box 715, Mecca 21955, Kingdom of Saudi Arabia; Science and Technology Unit, Umm Al-Qura University, P.O. Box 715, Mecca 21955, Kingdom of Saudi Arabia; The Regional Laboratory, Molecular Diagnostics Unit, Department of Molecular Biology, Ministry of Health (MOH), P.O. Box 6251, Mecca, Kingdom of Saudi Arabia; Department of Pathology and Lab Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON K1H 8M5, Canada",
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Abstract: 
The World Health Organization has declared the rapidly spreading coronavirus to be a global pandemic. The FDA is yet to approve a vaccine for human novel coronavirus. Here, we developed a peptide-based vaccine and used high-throughput screening by molecular dynamics simulation to identify T-cell- and ?-cell-recognized epitopes for producing specific antibodies against SARS-nCoV-2. We construct?~?12 P? antigenic epitope peptides to develop a more effective vaccine and identify specific antibodies. These epitope peptides selectively presented the best antigen presentation scores for both human pMHC class I and II alleles to develop a strong binding affinity. All antigens identified of SARS-nCoV-2 different proteins by each attached specific?~?17 L linker adaptor were used to construct a broad single peripheral peptide vaccine. It is expected to be highly antigenic with a minimum allergic effect. As a result of these exciting outcomes, expressing a vaccine using the intimated peptide was highly promising and positive to be highly proposed as epitope-based peptide vaccine of specific antibody against SARS-nCoV-2 by initiating T cells and ?-cells. An in vitro study for the proposed peptide-based vaccine is mostly recommended. Further clinical trials are required to check the efficacy of this vaccine.

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