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On-line Access: 2021-07-17
Received: 2021-02-21
Revision Accepted: 2021-06-04
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Porous hydrogel arrays forhepatoma cell spheroid formation anddrug resistance investigation
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XinLei, ChangminShao, XinShou, KeqingShi, LiangShi, YuanjinZhao. Porous hydrogel arrays forhepatoma cell spheroid formation anddrug resistance investigation[J]. Journal of Zhejiang University Science D, 2021, 4(4): 842-850.
@article{title="Porous hydrogel arrays forhepatoma cell spheroid formation anddrug resistance investigation",
author="XinLei, ChangminShao, XinShou, KeqingShi, LiangShi, YuanjinZhao",
journal="Journal of Zhejiang University Science D",
volume="4",
number="4",
pages="842-850",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-021-00141-8"
}
%0 Journal Article
%T Porous hydrogel arrays forhepatoma cell spheroid formation anddrug resistance investigation
%A XinLei
%A ChangminShao
%A XinShou
%A KeqingShi
%A LiangShi
%A YuanjinZhao
%J Journal of Zhejiang University SCIENCE D
%V 4
%N 4
%P 842-850
%@ 1869-1951
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-021-00141-8
TY - JOUR
T1 - Porous hydrogel arrays forhepatoma cell spheroid formation anddrug resistance investigation
A1 - XinLei
A1 - ChangminShao
A1 - XinShou
A1 - KeqingShi
A1 - LiangShi
A1 - YuanjinZhao
J0 - Journal of Zhejiang University Science D
VL - 4
IS - 4
SP - 842
EP - 850
%@ 1869-1951
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-021-00141-8
Abstract: drug resistance is one of the major obstacles in the drug therapy of cancers. Eforts in this area in pre-clinical research have
focused on developing novel platforms to evaluate and decrease drug resistance. In this paper, inspired by the structure of
hives where swarms live and breed, we propose porous hydrogel arrays with a uniform pore structure for the generation of
hepatoma cell spheroids and the investigation of drug resistance. The porous hydrogel arrays were fabricated using polyethylene glycol diacrylate (PEGDA) hydrogel to negatively replicate a well-designed template. Benefting from the elaborate
processing of the template, the prepared porous hydrogel arrays possessed a uniform pore structure. Due to their anti-adhesion
properties and the excellent biocompatibility of the PEGDA hydrogel, the hepatoma cells could form well-defned and uniform hepatoma cell spheroids in the porous hydrogel arrays. We found that the resistant hepatoma cell spheroids showed
more signifcant Lenvatinib resistance and a migratory phenotype compared with a two-dimensional (2D) cell culture, which
reveals the reason for the failure of most 2D cell-selected drugs for invivo applications. These features give such porous
hydrogel arrays promising application prospects in the investigation of tumor cell spheroid culture and invitro drug resistance.
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