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Bio-Design and Manufacturing  2023 Vol.6 No.3 P.268-283

http://doi.org/10.1007/s42242-022-00223-1


Development of a portable reflectance confocal microscope and its application in the noninvasive in vivo evaluation of mesenchymal stem cell-promoted cutaneous wound healing


Author(s):  Lixing Zhang, Xin Miao, Meijia Wang, Aihua Shi, Jingwen Wang, Zhonglin Ma, Yunhai Zhang, Jingzhong Zhang & Shuang Yu

Affiliation(s):  Suzhou Institute of Biomedical Engineering and Technology (SIBET), Chinese Academy of Sciences, Suzhou 215163, China; more

Corresponding email(s):   zhangjz@sibet.ac.cn, yush@sibet.ac.cn

Key Words:  Portable reflectance confocal microscope, Wound healing, Noninvasive optical imaging, Real-time in vivo visualization


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Lixing Zhang, Xin Miao, Meijia Wang, Aihua Shi, Jingwen Wang, Zhonglin Ma, Yunhai Zhang, Jingzhong Zhang & Shuang Yu. Development of a portable reflectance confocal microscope and its application in the noninvasive in vivo evaluation of mesenchymal stem cell-promoted cutaneous wound healing[J]. Journal of Zhejiang University Science D, 2023, 6(3): 268-283.

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author="Lixing Zhang, Xin Miao, Meijia Wang, Aihua Shi, Jingwen Wang, Zhonglin Ma, Yunhai Zhang, Jingzhong Zhang & Shuang Yu",
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Abstract: 
The process of wound healing is routinely evaluated by histological evaluation in the clinic, which may cause scarring and secondary injury. Reflectance confocal microscopy (RCM) represents a noninvasive, real-time imaging technique that allows in vivo evaluation of the skin. Traditional RCM was wide-probe-based, which limited its application on uneven and covered skin. In this study, we report the development of a portable reflectance confocal microscope (PRCM) in which all components were assembled in a handheld shell. Although the size and weight of the PRCM were reduced based on the use of a microelectromechanical system, the resolution was kept at 0.91 µm, and the field of view of the system was 343 μm×532 μm. When used in vivo, the PRCM was able to visualize cellular and nuclear morphology for both mouse and human skin. PRCM evaluations were then performed on wounds after topically applied mesenchymal stem cells (MSCs) or saline treatment. The PRCM allowed visualization of the formation of collagen bundles, re-epithelization from the wound edge to the wound bed, and hair follicle regeneration, which were consistent with histological evaluations. Therefore, we offer new insights into monitoring the effects of topically applied MSCs on the process of wound healing by using PRCM. This study illustrates that the newly developed PRCM represents a promising device for real-time, noninvasive monitoring of the dynamic process of wound healing, which demonstrates its potential to diagnose, monitor, or predict disease in clinical wound therapy.

中科院苏州生物医学工程技术研究所张丽星等 | 便携式反射共聚焦显微镜的研制及其在体无创评价间充质干细胞促进皮肤伤口愈合中的应用

本研究论文聚焦便携式反射共聚焦显微镜的研制及其在体无创评价间充质干细胞促进皮肤伤口愈合中的应用。临床上通常通过组织学评估伤口愈合的过程,但这可能会导致瘢痕和继发性损伤。反射共聚焦显微镜(RCM)是一种非侵入式的实时成像技术,可在体对皮肤结构进行评估。传统的RCM是基于宽探针的,这限制了它在凹凸不平和被覆盖皮肤上的应用。本研究中,我们设计并制造了一台便携式反射共聚焦显微镜(PRCM),其中PRCM中的所有组件都被组装在手持外壳中。通过采用微机电系统(MEMS)减小了PRCM的尺寸和重量,但分辨率保持在0.91 μm,系统的视场为343 μm×532 μm。当在体使用时,PRCM能够可视化小鼠和人类皮肤的细胞和核形态。利用PRCM对局部应用间充质干细胞(MSCs)或生理盐水治疗伤口的愈合过程进行评估,可以观测到胶原束的形成,从伤口边缘到伤口中央上皮细胞的形成,以及毛囊再生,这与组织学评估结果相一致。因此,我们为实时监测局部应用间充质干细胞对伤口愈合过程的影响提供了新的方法。这项研究表明,新开发的PRCM是一种有前途的、可实时、无创监测伤口动态愈合过程的设备,也显示出它在临床伤口治疗中具有诊断、监测或预测疾病的潜力。

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