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Bio-Design and Manufacturing  2016 Vol.-1 No.-1 P.

http://doi.org/10.1007/s42242-022-00246-X


Accelerated fracture healing by osteogenic Ti45Nb implants through the PI3K-Akt signaling pathway


Author(s):  Jia Tan, Jiaxin Li, Zhaoyang Ran, Junxiang Wu, Dinghao Luo, Bojun Cao, Liang Deng, Xiaoping Li, Wenbo Jiang, Kai Xie, Lei Wang, Yongqiang Hao

Affiliation(s):  Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; more

Corresponding email(s):   hyq_9hospital@hotmail.com, wanglei12041985@163.com, kai.xie@hotmail.com

Key Words:  Fracture healing, Ti45Nb alloy, PI3K-Akt pathway, Orthopedic implant


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Jia Tan,Jiaxin Li,Zhaoyang Ran,Junxiang Wu,Dinghao Luo,Bojun Cao,Liang Deng,Xiaoping Li,Wenbo Jiang,Kai Xie,Lei Wang,Yongqiang Hao. Accelerated fracture healing by osteogenic Ti45Nb implants through the PI3K-Akt signaling pathway[J]. Journal of Zhejiang University Science D, 2016, -1(-1): .

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%A Jia Tan
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%A Zhaoyang Ran
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%A Dinghao Luo
%A Bojun Cao
%A Liang Deng
%A Xiaoping Li
%A Wenbo Jiang
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%A Lei Wang
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A1 - Jia Tan
A1 - Jiaxin Li
A1 - Zhaoyang Ran
A1 - Junxiang Wu
A1 - Dinghao Luo
A1 - Bojun Cao
A1 - Liang Deng
A1 - Xiaoping Li
A1 - Wenbo Jiang
A1 - Kai Xie
A1 - Lei Wang
A1 - Yongqiang Hao
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PB - Zhejiang University Press & Springer
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DOI - 10.1007/s42242-022-00246-X


Abstract: 
The key to managing fracture is to achieve stable internal fixation, and currently, biologically and mechanically appropriate internal fixation devices are urgently needed. With excellent biocompatibility and corrosion resistance, titanium-niobium alloys have the potential to become a new generation of internal fixation materials for fractures. However, the role and mechanism of titanium-niobium alloys on promoting fracture healing are still undefined. Therefore, in this study, we systematically evaluated the bone-enabling properties of Ti45Nb via in vivo and in vitro experiments. In vitro, we found that Ti45Nb has an excellent ability to promote MC3T3-E1 cell adhesion and proliferation without obvious cytotoxicity. Alkaline phosphatase (ALP) activity and alizarin red staining and semiquantitative analysis showed that Ti45Nb enhanced the osteogenic differentiation of MC3T3-E1 cells compared to the Ti6Al4V control. In the polymerase chain reaction (PCR) experiment, the expression of osteogenic genes in the Ti45Nb group, such as ALP, OPN, OCN, Col-1 and Runx2, was significantly higher than that in the control group. Meanwhile, in the western blot (WB) experiment, the expression of osteogenic-related proteins in the Ti45Nb group was also significantly increased, and the expression of PI3K-Akt-related proteins was also higher, which indicated that Ti45Nb might promote fracture healing by activating the PI3k-Akt signaling pathway. In vivo, we found that Ti45Nb implants accelerated fracture healing compared to Ti6Al4V, and the biosafety of Ti45Nb was confirmed by histological evaluation. Furthermore, immunohistochemical staining confirmed that Ti45Nb may promote osteogenesis by upregulating the PI3K/Akt signaling pathway. Our study demonstrated that Ti45Nb exerts an excellent ability to promote fracture healing as well as enhance osteoblast differentiation by activating the PI3K/Akt signaling pathway, and its good biosafety has been confirmed, which indicates its clinical translation potential.

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